Restriction map for
pFN21AE5361
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI BstYI Bsu36I ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MflI
MluNI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 603 TCCGGA T^CCGGA
AclI 305 AACGTT AA^CGTT
Alw44I 508 GTGCAC G^TGCAC
AlwI 265 GATCC GGATC(4/5)
ApaLI 508 GTGCAC G^TGCAC
Asp700I 132 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 220 CTCGGG C^[CT]CG[GA]G
AvaII 9 GGTCC G^G[AT]CC
BanI 200 GGCACC G^G[CT][GA]CC
BcgI 620 GCACGTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 197 CCCGG CC^[GC]GG
BlpI 769 GCTGAGC GC^T[ACGT]AGC
BpmI 246 CTGGAG CTGGAG(16/14)
Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC
BsaWI 603 TCCGGA [AT]^CCGG[AT]
BseAI 603 TCCGGA T^CCGGA
BseRI 151 CTCCTC GAGGAG(10/8)
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsiWI 17 CGTACG C^GTACG
BsoBI 220 CTCGGG C^[CT]CG[GA]G
BspEI 603 TCCGGA T^CCGGA
BspHI 780 TCATGA T^CATGA
BsrDI 765 CATTGC GCAATG(2/0)
BsrI 105 ACTGG ACTGG(1/-1)
BstF5I 606 GGATG GGATG(2/0)
CelII 769 GCTGAGC GC^T[ACGT]AGC
DraII 146 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 871 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 209 CCGCGG C^C[GA][CT]GG
EaeI 113 CGGCCG [CT]^GGCC[GA]
EagI 113 CGGCCG C^GGCCG
EclXI 113 CGGCCG C^GGCCG
Eco52I 113 CGGCCG C^GGCCG
EcoNI 153 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 146 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 35 CCCGC CCCGC(4/6)
FokI 606 GGATG GGATG(9/13)
HgaI 181 GACGC GACGC(5/10)
KspI 209 CCGCGG CCGC^GG
MboII 702 TCTTC GAAGA(8/7)
MluI 182 ACGCGT A^CGCGT
MroI 603 TCCGGA T^CCGGA
MslI 420 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 209 CCGCGG C[AC]G^C[GT]G
NciI 197 CCCGG CC^[GC]GG
NlaIV 200 GGCACC GG[ACGT]^[ACGT]CC
Psp1406I 305 AACGTT AA^CGTT
RcaI 780 TCATGA T^CATGA
SacII 209 CCGCGG CCGC^GG
SfaNI 607 GATGC GCATC(5/9)
TaqI 629 TCGA T^CGA
TthHB8I 629 TCGA T^CGA
XmnI 132 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 743 GTATAC GT^[AC][GT]AC
903 GTATAC
AcsI 350 GAATTC [GA]^AATT[CT]
726 GAATTC
AfaI 18 GTAC GT^AC
331 GTAC
AflIII 182 ACGCGT A^C[GA][CT]GT
298 ACGTGT
ApoI 350 GAATTC [GA]^AATT[CT]
726 GAATTC
BfaI 335 CTAG C^TAG
640 CTAG
BsmAI 251 GAGAC GTCTC(1/5)
644 GAGAC
BsrFI 76 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
Bst1107I 743 GTATAC GTA^TAC
903 GTATAC
BstAPI 410 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
820 GCACAGTTTGC
BstZ17I 743 GTATAC GTA^TAC
903 GTATAC
CfoI 38 GCGC GCG^C
164 GCGC
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
DpnII 265 GATC ^GATC
515 GATC
EcoRI 350 GAATTC G^AATTC
726 GAATTC
HhaI 38 GCGC GCG^C
164 GCGC
HinP1I 38 GCGC G^CGC
164 GCGC
MaeI 335 CTAG C^TAG
640 CTAG
MboI 265 GATC ^GATC
515 GATC
MseI 289 TTAA T^TAA
721 TTAA
NaeI 76 GCCGGC GCC^GGC
115 GCCGGC
NdeII 265 GATC ^GATC
515 GATC
NgoMIV 76 GCCGGC G^CCGGC
115 GCCGGC
NspI 683 ACATGC [GA]CATG^[CT]
829 GCATGT
PleI 671 GACTC GAGTC(4/5)
584 GAGTC
RsaI 18 GTAC GT^AC
331 GTAC
Sau3AI 265 GATC ^GATC
515 GATC
SmlI 154 CTCAAG C^T[CT][GA]AG
591 CTTGAG
TfiI 472 GATTC G^A[AT]TC
699 GATTC
Tru9I 289 TTAA T^TAA
721 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI BstUI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MnlI MspI
MvaI MvnI MwoI NlaIII Sau96I
ScrFI TseI Tsp45I Tsp509I