Restriction map for
pFN21AE6256
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspHI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HinfI HpaI
HphI KasI KpnI MaeII MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 39 CGCG CG^CG
AccIII 200 TCCGGA T^CCGGA
Aor51HI 489 AGCGCT AGC^GCT
ApaI 61 GGGCCC GGGCC^C
Asp700I 435 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 70 CTCGAG C^[CT]CG[GA]G
BanII 61 GGGCCC G[GA]GC[CT]^C
BbsI 316 GTCTTC GAAGAC(2/6)
BcnI 59 CCGGG CC^[GC]GG
BfaI 550 CTAG C^TAG
BglII 437 AGATCT A^GATCT
BlpI 607 GCTGAGC GC^T[ACGT]AGC
BmyI 61 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 607 GCTGAGC GC^T[ACGT]AGC
BpuAI 316 GTCTTC GAAGAC(2/6)
BsaI 55 GAGACC GGTCTC(1/5)
BsaWI 200 TCCGGA [AT]^CCGG[AT]
BseAI 200 TCCGGA T^CCGGA
BsgI 430 GTGCAG GTGCAG(16/14)
BsmAI 55 GAGAC GTCTC(1/5)
BsoBI 70 CTCGAG C^[CT]CG[GA]G
Bsp1286I 61 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 590 TGTACA T^GTACA
BspDI 571 ATCGAT AT^CGAT
BspEI 200 TCCGGA T^CCGGA
BspMI 86 ACCTGC ACCTGC(4/8)
BsrBI 343 GAGCGG CCGCTC(-3/-3)
BsrGI 590 TGTACA T^GTACA
BstUI 39 CGCG CG^CG
CelII 607 GCTGAGC GC^T[ACGT]AGC
ClaI 571 ATCGAT AT^CGAT
DdeI 608 CTGAG C^T[ACGT]AG
EaeI 11 TGGCCG [CT]^GGCC[GA]
EarI 544 CTCTTC CTCTTC(1/4)
Eco47III 489 AGCGCT AGC^GCT
Ksp632I 544 CTCTTC CTCTTC(1/4)
KspI 38 CCGCGG CCGC^GG
MaeI 550 CTAG C^TAG
MaeIII 511 GTTAC ^GT[ACGT]AC
MroI 200 TCCGGA T^CCGGA
MseI 614 TTAA T^TAA
MslI 594 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 39 CGCG CG^CG
NciI 59 CCGGG CC^[GC]GG
NcoI 417 CCATGG C^CATGG
NlaIII 418 CATG CATG^
PaeR7I 70 CTCGAG C^TCGAG
PpuMI 112 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 208 CTGCAG CTGCA^G
SacII 38 CCGCGG CCGC^GG
SfaNI 329 GCATC GCATC(5/9)
SfcI 208 CTGCAG C^T[GA][CT]AG
SspBI 590 TGTACA T^GTACA
Tru9I 614 TTAA T^TAA
XhoI 70 CTCGAG C^TCGAG
XmnI 435 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 171 AGCT AG^CT
611 AGCT
AlwI 537 GATCC GGATC(4/5)
425 GGATC
AvaII 113 GGTCC G^G[AT]CC
203 GGACC
BglI 268 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
382 GCCACCAAGGC
BseRI 517 GAGGAG GAGGAG(10/8)
520 GAGGAG
BsrI 273 ACTGG ACTGG(1/-1)
118 CCAGT
BstYI 437 AGATCT [GA]^GATC[CT]
536 AGATCC
Cac8I 9 GCTGGC GC[ACGT]^[ACGT]GC
36 GCCCGC
DraII 61 GGGCCCT [GA]G^G[ACGT]CC[CT]
112 GGGTCCC
DsaI 38 CCGCGG C^C[GA][CT]GG
417 CCATGG
EcoO109I 61 GGGCCCT [GA]G^G[ACGT]CC[CT]
112 GGGTCCC
FauI 37 CCCGC CCCGC(4/6)
345 GCGGG
HapII 59 CCGG C^CGG
201 CCGG
HincII 376 GTCAAC GT[CT]^[GA]AC
553 GTCAAC
HindII 376 GTCAAC GT[CT]^[GA]AC
553 GTCAAC
HpaII 59 CCGG C^CGG
201 CCGG
MflI 437 AGATCT [GA]^GATC[CT]
536 AGATCC
MspA1I 31 CCGCTG C[AC]G^C[GT]G
38 CCGCGG
MspI 59 CCGG C^CGG
201 CCGG
SmlI 70 CTCGAG C^T[CT][GA]AG
406 CTCAAG
StuI 297 AGGCCT AGG^CCT
389 AGGCCT
XhoII 437 AGATCT [GA]^GATC[CT]
536 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI BbvI BpmI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI CfoI Cfr13I DpnII EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I ItaI MboI MboII
MnlI MvaI MwoI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
TaqI TseI TspRI TthHB8I