Restriction map for
pFN21AB8516
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AspI AvrII BamHI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaWI BseAI BsiEI BsiWI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CpoI DraI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MaeI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2475 GGTACC G^GTACC
AccI 1858 GTCTAC GT^[AC][GT]AC
AcsI 1620 GAATTC [GA]^AATT[CT]
AhdI 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1474 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2253 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2234 AGCGCT AGC^GCT
ApoI 1620 GAATTC [GA]^AATT[CT]
Asp700I 2259 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2475 GGTACC G^GTACC
AspEI 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 200 TGCGCA TGC^GCA
BbrPI 1275 CACGTG CAC^GTG
BbvCI 418 CCTCAGC CCTCAGC(-5/-2)
BsaBI 1146 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1761 GAATGC GAATGC(1/-1)
Bsp1407I 1313 TGTACA T^GTACA
BspDI 247 ATCGAT AT^CGAT
BsrGI 1313 TGTACA T^GTACA
BstAPI 2321 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2253 CCTGAGG CC^T[ACGT]AGG
ClaI 247 ATCGAT AT^CGAT
DraIII 754 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2234 AGCGCT AGC^GCT
Eco81I 2253 CCTGAGG CC^T[ACGT]AGG
EcoRI 1620 GAATTC G^AATTC
FspI 200 TGCGCA TGC^GCA
HincII 901 GTCAAC GT[CT]^[GA]AC
HindII 901 GTCAAC GT[CT]^[GA]AC
KpnI 2475 GGTACC GGTAC^C
MamI 1146 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 955 TCGCGA TCG^CGA
PmaCI 1275 CACGTG CAC^GTG
PmlI 1275 CACGTG CAC^GTG
PstI 2203 CTGCAG CTGCA^G
PvuII 1648 CAGCTG CAG^CTG
SfiI 1716 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1051 CTCAAG C^T[CT][GA]AG
SphI 975 GCATGC GCATG^C
SspBI 1313 TGTACA T^GTACA
Tsp509I 1621 AATT ^AATT
XmnI 2259 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1274 ACACGT A^C[GA][CT]GT
1276 ACGTGT
ApaI 31 GGGCCC GGGCC^C
292 GGGCCC
BbeI 217 GGCGCC GGCGC^C
944 GGCGCC
BglI 167 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1717 GCCTACCTGGC
BlpI 46 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
BsaAI 757 TACGTG [CT]AC^GT[GA]
1275 CACGTG
BsmBI 1404 CGTCTC CGTCTC(1/5)
1683 CGTCTC
BsrBI 2268 CCGCTC CCGCTC(-3/-3)
2608 CCGCTC
BstYI 1151 GGATCT [GA]^GATC[CT]
1304 AGATCC
CelII 46 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
EcoNI 582 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2253 CCTGAGGAAGG
HgaI 2602 GACGC GACGC(5/10)
741 GCGTC
KasI 217 GGCGCC G^GCGCC
944 GGCGCC
MflI 1151 GGATCT [GA]^GATC[CT]
1304 AGATCC
MseI 680 TTAA T^TAA
2021 TTAA
NarI 217 GGCGCC GG^CGCC
944 GGCGCC
NcoI 308 CCATGG C^CATGG
1442 CCATGG
SacI 49 GAGCTC GAGCT^C
2298 GAGCTC
SapI 1551 GCTCTTC GCTCTTC(1/4)
1866 GCTCTTC
SfcI 1803 CTATAG C^T[GA][CT]AG
2203 CTGCAG
SfoI 217 GGCGCC GGC^GCC
944 GGCGCC
Tru9I 680 TTAA T^TAA
2021 TTAA
XhoII 1151 GGATCT [GA]^GATC[CT]
1304 AGATCC
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI