Restriction map for
pFN21AE3875
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvrII BbeI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwNI 36 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 9 AGCGCT AGC^GCT
AspI 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 686 TGCGCA TGC^GCA
BalI 56 TGGCCA TGG^CCA
BamHI 70 GGATCC G^GATCC
BanI 637 GGCACC G^G[CT][GA]CC
BbrPI 261 CACGTG CAC^GTG
BbsI 483 GAAGAC GAAGAC(2/6)
BfaI 574 CTAG C^TAG
BglI 454 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 205 CTGGAG CTGGAG(16/14)
BpuAI 483 GAAGAC GAAGAC(2/6)
BsmBI 468 CGTCTC CGTCTC(1/5)
BspHI 401 TCATGA T^CATGA
BstAPI 231 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 59 CCACTTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 325 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 440 CCATGG C^C[GA][CT]GG
EarI 847 GAAGAG CTCTTC(1/4)
Eco47III 9 AGCGCT AGC^GCT
EcoNI 563 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 949 GAATTC G^AATTC
FspI 686 TGCGCA TGC^GCA
Ksp632I 847 GAAGAG CTCTTC(1/4)
MaeI 574 CTAG C^TAG
MaeIII 253 GTGAC ^GT[ACGT]AC
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MseI 1015 TTAA T^TAA
NcoI 440 CCATGG C^CATGG
PflFI 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 261 CACGTG CAC^GTG
PmlI 261 CACGTG CAC^GTG
PpuMI 163 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 401 TCATGA T^CATGA
SbfI 177 CCTGCAGG CCTGCA^GG
SexAI 248 ACCAGGT A^CC[AT]GGT
Sse8387I 177 CCTGCAGG CCTGCA^GG
Tru9I 1015 TTAA T^TAA
Tsp45I 253 GTGAC ^GT[GC]AC
Tth111I 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 706 AAATTC [GA]^AATT[CT]
949 GAATTC
AflIII 260 ACACGT A^C[GA][CT]GT
465 ACACGT
ApoI 706 AAATTC [GA]^AATT[CT]
949 GAATTC
BsaAI 261 CACGTG [CT]AC^GT[GA]
364 TACGTG
BseRI 712 GAGGAG GAGGAG(10/8)
754 GAGGAG
BsgI 229 CTGCAC GTGCAG(16/14)
381 CTGCAC
BsmAI 838 GAGAC GTCTC(1/5)
469 GTCTC
BssSI 346 CACGAG CACGAG(-5/-1)
823 CTCGTG
BstYI 70 GGATCC [GA]^GATC[CT]
446 AGATCC
DdeI 969 CTCAG C^T[ACGT]AG
1029 CTCAG
DraII 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
680 AGGCCCT
EaeI 56 TGGCCA [CT]^GGCC[GA]
452 CGGCCA
EcoO109I 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
680 AGGCCCT
EcoT14I 371 CCAAGG C^C[AT][AT]GG
440 CCATGG
HaeII 9 AGCGCT [GA]GCGC^[CT]
277 AGCGCC
HphI 252 GGTGA GGTGA(8/7)
599 TCACC
MflI 70 GGATCC [GA]^GATC[CT]
446 AGATCC
PstI 178 CTGCAG CTGCA^G
868 CTGCAG
SacI 208 GAGCTC GAGCT^C
349 GAGCTC
SfaNI 237 GATGC GCATC(5/9)
503 GCATC
SfcI 178 CTGCAG C^T[GA][CT]AG
868 CTGCAG
StyI 371 CCAAGG C^C[AT][AT]GG
440 CCATGG
XhoII 70 GGATCC [GA]^GATC[CT]
446 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
TseI Tsp509I TspRI TthHB8I