Restriction map for
pFN21AE1884
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HphI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Van91I
XbaI XcmI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 322 GTAGAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AfaI 646 GTAC GT^AC
AflII 882 CTTAAG C^TTAAG
AlwI 1003 GATCC GGATC(4/5)
Asp700I 124 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 827 GTGCTC G[AT]GC[AT]^C
BclI 531 TGATCA T^GATCA
BfaI 643 CTAG C^TAG
BfrI 882 CTTAAG C^TTAAG
BglII 603 AGATCT A^GATCT
BpmI 609 CTCCAG CTGGAG(16/14)
BseRI 1109 GAGGAG GAGGAG(10/8)
BsiHKAI 827 GTGCTC G[AT]GC[AT]^C
BsiYI 457 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 457 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 423 GGGAC GGGAC(10/14)
BsmI 1130 GCATTC GAATGC(1/-1)
BstAPI 820 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 15 CGCG CG^CG
BstXI 569 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 603 AGATCT [GA]^GATC[CT]
DraI 304 TTTAAA TTT^AAA
EarI 1074 CTCTTC CTCTTC(1/4)
FauI 16 GCGGG CCCGC(4/6)
FbaI 531 TGATCA T^GATCA
HindIII 44 AAGCTT A^AGCTT
Ksp632I 1074 CTCTTC CTCTTC(1/4)
MaeI 643 CTAG C^TAG
MflI 603 AGATCT [GA]^GATC[CT]
MslI 523 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 514 CAGCTG C[AC]G^C[GT]G
MvnI 15 CGCG CG^CG
PaeR7I 839 CTCGAG C^TCGAG
PvuII 514 CAGCTG CAG^CTG
RsaI 646 GTAC GT^AC
SfcI 949 CTACAG C^T[GA][CT]AG
SmaI 420 CCCGGG CCC^GGG
TaqI 840 TCGA T^CGA
TthHB8I 840 TCGA T^CGA
XhoI 839 CTCGAG C^TCGAG
XhoII 603 AGATCT [GA]^GATC[CT]
XmaCI 420 CCCGGG C^CCGGG
XmaI 420 CCCGGG C^CCGGG
XmnI 124 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 7 GCGG CCGC(-3/-1)
16 GCGG
AlwNI 140 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
344 CAGAAGCTG
AspI 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1152 GACCCAGTC
AvaI 420 CCCGGG C^[CT]CG[GA]G
839 CTCGAG
BanII 499 GGGCTC G[GA]GC[CT]^C
842 GAGCCC
BsoBI 420 CCCGGG C^[CT]CG[GA]G
839 CTCGAG
HapII 421 CCGG C^CGG
593 CCGG
HpaII 421 CCGG C^CGG
593 CCGG
MspI 421 CCGG C^CGG
593 CCGG
MwoI 7 GCGGAGAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
820 GCAAGATGTGC
NdeI 523 CATATG CA^TATG
1022 CATATG
PflFI 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1152 GACCCAGTC
PleI 366 GACTC GAGTC(4/5)
241 GAGTC
SfaNI 967 GATGC GCATC(5/9)
447 GCATC
TfiI 774 GATTC G^A[AT]TC
1114 GAATC
Tth111I 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1152 GACCCAGTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BcnI BmyI BsaJI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HinfI ItaI MaeII MboI MboII
MnlI MseI MvaI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SmlI Tru9I TseI Tsp509I TspRI