################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MslI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 220 GCGG CCGC(-3/-1) AcsI 147 AAATTC [GA]^AATT[CT] AflIII 4 ACGTGT A^C[GA][CT]GT ApoI 147 AAATTC [GA]^AATT[CT] Asp700I 343 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 506 TGGCCA TGG^CCA BbsI 483 GAAGAC GAAGAC(2/6) BclI 657 TGATCA T^GATCA BmyI 512 GGGCAC G[AGT]GC[ACT]^C BpuAI 483 GAAGAC GAAGAC(2/6) BseRI 178 GAGGAG GAGGAG(10/8) BsmI 687 GAATGC GAATGC(1/-1) Bsp1286I 512 GGGCAC G[AGT]GC[ACT]^C BstF5I 608 CATCC GGATG(2/0) DraI 675 TTTAAA TTT^AAA DraIII 641 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 506 TGGCCA [CT]^GGCC[GA] EarI 293 CTCTTC CTCTTC(1/4) FbaI 657 TGATCA T^GATCA FokI 608 CATCC GGATG(9/13) HphI 732 TCACC GGTGA(8/7) Ksp632I 293 CTCTTC CTCTTC(1/4) MaeII 4 ACGT A^CGT MluNI 506 TGGCCA TGG^CCA MscI 506 TGGCCA TGG^CCA NdeI 707 CATATG CA^TATG NlaIV 180 GGAGCC GG[ACGT]^[ACGT]CC PflMI 498 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 599 GACTC GAGTC(4/5) PstI 256 CTGCAG CTGCA^G ScaI 86 AGTACT AGT^ACT SspI 241 AATATT AAT^ATT TfiI 355 GAATC G^A[AT]TC Van91I 498 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 343 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 369 TCCGGA T^CCGGA 554 TCCGGA AfaI 87 GTAC GT^AC 283 GTAC BbvI 155 GCTGC GCAGC(8/12) 217 GCTGC BglII 78 AGATCT A^GATCT 629 AGATCT BpmI 298 CTCCAG CTGGAG(16/14) 74 CTGGAG BsaJI 509 CCAGGG C^C[ACGT][ACGT]GG 618 CCTGGG BsaWI 369 TCCGGA [AT]^CCGG[AT] 554 TCCGGA BseAI 369 TCCGGA T^CCGGA 554 TCCGGA BspEI 369 TCCGGA T^CCGGA 554 TCCGGA BstYI 78 AGATCT [GA]^GATC[CT] 629 AGATCT Fnu4HI 155 GCTGC GC^[ACGT]GC 217 GCTGC HaeIII 507 GGCC GG^CC 585 GGCC HapII 370 CCGG C^CGG 555 CCGG HinfI 355 GAATC G^A[ACGT]TC 599 GACTC HpaII 370 CCGG C^CGG 555 CCGG ItaI 155 GCTGC GC^[ACGT]GC 217 GCTGC MflI 78 AGATCT [GA]^GATC[CT] 629 AGATCT MroI 369 TCCGGA T^CCGGA 554 TCCGGA MspA1I 153 CAGCTG C[AC]G^C[GT]G 277 CAGCTG MspI 370 CCGG C^CGG 555 CCGG PvuII 153 CAGCTG CAG^CTG 277 CAGCTG RsaI 87 GTAC GT^AC 283 GTAC SfcI 173 CTACAG C^T[GA][CT]AG 256 CTGCAG TseI 155 GCTGC G^C[AT]GC 217 GCTGC XhoII 78 AGATCT [GA]^GATC[CT] 629 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BsiYI BslI BsrI BssKI BstNI DdeI DpnII EcoRII MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI SfaNI Tru9I Tsp509I TspRI