Restriction map for
pFN21AB6166
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI HinP1I
HpaI KasI KpnI KspI MamI
MluI MroI MslI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 365 GACGTC GACGT^C
AcyI 365 GACGTC G[GA]^CG[CT]C
AflII 900 CTTAAG C^TTAAG
AflIII 1116 ACATGT A^C[GA][CT]GT
Alw44I 1168 GTGCAC G^TGCAC
AlwI 451 GGATC GGATC(4/5)
ApaLI 1168 GTGCAC G^TGCAC
AseI 469 ATTAAT AT^TAAT
AsnI 469 ATTAAT AT^TAAT
AspI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1020 GAGCTC G[GA]GC[CT]^C
BciVI 1486 GGATAC GTATCC(6/5)
BclI 105 TGATCA T^GATCA
BcnI 196 CCGGG CC^[GC]GG
BfrI 900 CTTAAG C^TTAAG
BglII 858 AGATCT A^GATCT
BsaHI 365 GACGTC G[GA]^CG[CT]C
BsaI 1714 GGTCTC GGTCTC(1/5)
BsgI 269 CTGCAC GTGCAG(16/14)
BsmI 1680 GAATGC GAATGC(1/-1)
Bsp1407I 1047 TGTACA T^GTACA
BspHI 722 TCATGA T^CATGA
BspLU11I 1116 ACATGT A^CATGT
BsrDI 905 GCAATG GCAATG(2/0)
BsrGI 1047 TGTACA T^GTACA
BssSI 578 CACGAG CACGAG(-5/-1)
BstAPI 1007 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 858 AGATCT [GA]^GATC[CT]
DraI 409 TTTAAA TTT^AAA
DraII 632 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1519 CTCTTC CTCTTC(1/4)
EcoO109I 632 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1591 GAATTC G^AATTC
FauI 1030 GCGGG CCCGC(4/6)
FbaI 105 TGATCA T^GATCA
HapII 196 CCGG C^CGG
Hin1I 365 GACGTC G[GA]^CG[CT]C
HpaII 196 CCGG C^CGG
Ksp632I 1519 CTCTTC CTCTTC(1/4)
MaeII 366 ACGT A^CGT
MfeI 1365 CAATTG C^AATTG
MflI 858 AGATCT [GA]^GATC[CT]
MspI 196 CCGG C^CGG
MunI 1365 CAATTG C^AATTG
NciI 196 CCGGG CC^[GC]GG
NspI 1116 ACATGT [GA]CATG^[CT]
PflFI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 632 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 469 ATTAAT AT^TAAT
PvuII 758 CAGCTG CAG^CTG
RcaI 722 TCATGA T^CATGA
SacI 1020 GAGCTC GAGCT^C
ScaI 1515 AGTACT AGT^ACT
SmlI 900 CTTAAG C^T[CT][GA]AG
SspBI 1047 TGTACA T^GTACA
SspI 727 AATATT AAT^ATT
Tth111I 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 858 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 313 GGACC G^G[AT]CC
633 GGACC
BalI 176 TGGCCA TGG^CCA
815 TGGCCA
BbsI 1370 GTCTTC GAAGAC(2/6)
1382 GTCTTC
BfaI 1249 CTAG C^TAG
1543 CTAG
BpuAI 1370 GTCTTC GAAGAC(2/6)
1382 GTCTTC
BsaJI 117 CCAAGG C^C[ACGT][ACGT]GG
309 CCAAGG
BsiYI 304 CCAGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1316 CCCAGTGCTGG
BslI 304 CCAGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1316 CCCAGTGCTGG
BsmAI 350 GAGAC GTCTC(1/5)
1715 GTCTC
EaeI 176 TGGCCA [CT]^GGCC[GA]
815 TGGCCA
EcoT14I 117 CCAAGG C^C[AT][AT]GG
309 CCAAGG
HindIII 897 AAGCTT A^AGCTT
1280 AAGCTT
MaeI 1249 CTAG C^TAG
1543 CTAG
MluNI 176 TGGCCA TGG^CCA
815 TGGCCA
MscI 176 TGGCCA TGG^CCA
815 TGGCCA
MspA1I 489 CCGCTG C[AC]G^C[GT]G
758 CAGCTG
NlaIV 1095 GGGGCC GG[ACGT]^[ACGT]CC
1767 GGAGCC
StyI 117 CCAAGG C^C[AT][AT]GG
309 CCAAGG
TaqI 447 TCGA T^CGA
1228 TCGA
Tsp45I 94 GTGAC ^GT[GC]AC
1799 GTCAC
TthHB8I 447 TCGA T^CGA
1228 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BmyI BpmI BsiHKAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI