Restriction map for
pFN21AE6571
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# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvaI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 25 CGCG CG^CG
AlwNI 13 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 15 GGTCC G^G[AT]CC
BamHI 410 GGATCC G^GATCC
BfaI 389 CTAG C^TAG
BsaI 55 GGTCTC GGTCTC(1/5)
BsgI 10 GTGCAG GTGCAG(16/14)
BsmAI 56 GTCTC GTCTC(1/5)
BspMI 12 GCAGGT ACCTGC(4/8)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BsrI 352 ACTGG ACTGG(1/-1)
BstUI 25 CGCG CG^CG
BstYI 410 GGATCC [GA]^GATC[CT]
Cac8I 26 GCGGGC GC[ACGT]^[ACGT]GC
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
Cfr13I 15 GGTCC G^G[ACGT]CC
DraII 14 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 48 CCACGG C^C[GA][CT]GG
EcoO109I 14 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 26 GCGGG CCCGC(4/6)
HapII 44 CCGG C^CGG
HinfI 399 GATTC G^A[ACGT]TC
HpaII 44 CCGG C^CGG
MaeI 389 CTAG C^TAG
MaeII 347 ACGT A^CGT
MflI 410 GGATCC [GA]^GATC[CT]
MspI 44 CCGG C^CGG
MvnI 25 CGCG CG^CG
MwoI 258 GCATCGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 240 CATG CATG^
PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 31 CTGCAG CTGCA^G
Sau96I 15 GGTCC G^G[ACGT]CC
SfcI 31 CTGCAG C^T[GA][CT]AG
TfiI 399 GATTC G^A[AT]TC
XhoII 410 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 26 GCGG CCGC(-3/-1)
53 GCGG
AcsI 319 AAATTC [GA]^AATT[CT]
340 AAATTC
AfaI 107 GTAC GT^AC
373 GTAC
ApoI 319 AAATTC [GA]^AATT[CT]
340 AAATTC
BanI 243 GGTGCC G^G[CT][GA]CC
355 GGCACC
BbsI 121 GAAGAC GAAGAC(2/6)
148 GAAGAC
BpuAI 121 GAAGAC GAAGAC(2/6)
148 GAAGAC
BsaJI 48 CCACGG C^C[ACGT][ACGT]GG
60 CCGAGG
BstF5I 225 GGATG GGATG(2/0)
231 GGATG
EaeI 45 CGGCCA [CT]^GGCC[GA]
168 TGGCCG
FokI 225 GGATG GGATG(9/13)
231 GGATG
HaeIII 46 GGCC GG^CC
169 GGCC
HphI 41 TCACC GGTGA(8/7)
174 TCACC
MaeIII 173 GTCAC ^GT[ACGT]AC
349 GTGAC
RsaI 107 GTAC GT^AC
373 GTAC
SfaNI 94 GATGC GCATC(5/9)
258 GCATC
Tsp45I 173 GTCAC ^GT[GC]AC
349 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AlwI BbvI DpnII Fnu4HI ItaI
MboI MboII MnlI NdeII NlaIV
Sau3AI TseI Tsp509I