Restriction map for
pFN21AE5558
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MluI MroI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TspRI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AflII 1102 CTTAAG C^TTAAG
AspHI 1076 GAGCTC G[AT]GC[AT]^C
BanII 1076 GAGCTC G[GA]GC[CT]^C
BbvI 290 GCAGC GCAGC(8/12)
BfaI 848 CTAG C^TAG
BfrI 1102 CTTAAG C^TTAAG
BglII 351 AGATCT A^GATCT
BmyI 1076 GAGCTC G[AGT]GC[ACT]^C
BpmI 16 CTGGAG CTGGAG(16/14)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaJI 272 CCCTGG C^C[ACGT][ACGT]GG
BseRI 166 GAGGAG GAGGAG(10/8)
BsgI 235 GTGCAG GTGCAG(16/14)
BsiHKAI 1076 GAGCTC G[AT]GC[AT]^C
BsmBI 5 CGTCTC CGTCTC(1/5)
BsmFI 497 GTCCC GGGAC(10/14)
Bsp1286I 1076 GAGCTC G[AGT]GC[ACT]^C
BspDI 832 ATCGAT AT^CGAT
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrI 1071 ACTGG ACTGG(1/-1)
BssKI 273 CCTGG ^CC[ACGT]GG
BstNI 273 CCTGG CC^[AT]GG
Cac8I 1030 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 35 GCGC GCG^C
ClaI 832 ATCGAT AT^CGAT
EcoRI 401 GAATTC G^AATTC
EcoRII 273 CCTGG ^CC[AT]GG
HaeII 34 GGCGCT [GA]GCGC^[CT]
HapII 73 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
HhaI 35 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 35 GCGC G^CGC
HpaII 73 CCGG C^CGG
HphI 853 GGTGA GGTGA(8/7)
MaeI 848 CTAG C^TAG
MfeI 983 CAATTG C^AATTG
MspA1I 1085 CAGCTG C[AC]G^C[GT]G
MspI 73 CCGG C^CGG
MunI 983 CAATTG C^AATTG
MvaI 273 CCTGG CC^[AT]GG
NspI 421 GCATGT [GA]CATG^[CT]
PvuII 1085 CAGCTG CAG^CTG
SacI 1076 GAGCTC GAGCT^C
ScrFI 273 CCTGG CC^[ACGT]GG
SfaNI 1034 GCATC GCATC(5/9)
SmlI 1102 CTTAAG C^T[CT][GA]AG
SspI 634 AATATT AAT^ATT
TseI 290 GCAGC G^C[AT]GC
Tsp45I 425 GTGAC ^GT[GC]AC
XcmI 152 CCAGAGGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 21 GCGG CCGC(-3/-1)
885 GCGG
AcsI 49 GAATTT [GA]^AATT[CT]
401 GAATTC
AfaI 305 GTAC GT^AC
397 GTAC
AlwI 325 GATCC GGATC(4/5)
505 GGATC
AocI 970 CCTAAGG CC^T[ACGT]AGG
994 CCTAAGG
ApoI 49 GAATTT [GA]^AATT[CT]
401 GAATTC
BalI 226 TGGCCA TGG^CCA
1028 TGGCCA
BbsI 315 GAAGAC GAAGAC(2/6)
479 GAAGAC
BpuAI 315 GAAGAC GAAGAC(2/6)
479 GAAGAC
BsiYI 25 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTTTTAGTGG
BslI 25 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTTTTAGTGG
BsmAI 936 GAGAC GTCTC(1/5)
6 GTCTC
BstF5I 640 CATCC GGATG(2/0)
411 GGATG
BstYI 351 AGATCT [GA]^GATC[CT]
505 GGATCT
Bsu36I 970 CCTAAGG CC^T[ACGT]AGG
994 CCTAAGG
DdeI 971 CTAAG C^T[ACGT]AG
995 CTAAG
Eco81I 970 CCTAAGG CC^T[ACGT]AGG
994 CCTAAGG
FokI 640 CATCC GGATG(9/13)
411 GGATG
HindIII 660 AAGCTT A^AGCTT
1001 AAGCTT
HinfI 88 GAATC G^A[ACGT]TC
691 GATTC
MflI 351 AGATCT [GA]^GATC[CT]
505 GGATCT
MluNI 226 TGGCCA TGG^CCA
1028 TGGCCA
MscI 226 TGGCCA TGG^CCA
1028 TGGCCA
MslI 259 CATGTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
742 CACAAAAATG
RsaI 305 GTAC GT^AC
397 GTAC
TaqI 284 TCGA T^CGA
833 TCGA
TfiI 88 GAATC G^A[AT]TC
691 GATTC
TthHB8I 284 TCGA T^CGA
833 TCGA
XhoII 351 AGATCT [GA]^GATC[CT]
505 GGATCT
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AvaII Cfr13I DpnII EaeI
Fnu4HI HaeIII ItaI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I Tru9I
Tsp509I