Restriction map for
pFN21AE9441
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbrPI BciVI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I FseI FspI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 2119 GGCGCC G[GA]^CG[CT]C
AflIII 381 ACACGT A^C[GA][CT]GT
AhdI 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2672 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 489 CCTGAGG CC^T[ACGT]AGG
Asp700I 1387 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2119 GGCGCC GGCGC^C
BcgI 248 CGAATCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1676 TGATCA T^GATCA
BlpI 1191 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1191 GCTTAGC GC^T[ACGT]AGC
BsaAI 382 CACGTA [CT]AC^GT[GA]
BsaHI 2119 GGCGCC G[GA]^CG[CT]C
BsaI 2261 GAGACC GGTCTC(1/5)
BsgI 1615 GTGCAG GTGCAG(16/14)
Bsp1407I 1760 TGTACA T^GTACA
BspHI 2213 TCATGA T^CATGA
BsrGI 1760 TGTACA T^GTACA
BstAPI 402 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2604 GGTAACC G^GT[ACGT]ACC
BstPI 2604 GGTAACC G^GT[ACGT]ACC
Bsu36I 489 CCTGAGG CC^T[ACGT]AGG
CelII 1191 GCTTAGC GC^T[ACGT]AGC
CfoI 2120 GCGC GCG^C
DraI 720 TTTAAA TTT^AAA
DraII 1635 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 2084 TGGCCG [CT]^GGCC[GA]
Eam1105I 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 489 CCTGAGG CC^T[ACGT]AGG
EcoNI 1627 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1635 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2604 GGTAACC G^GT[ACGT]ACC
EcoRI 742 GAATTC G^AATTC
EcoRV 1377 GATATC GAT^ATC
EcoT22I 1247 ATGCAT ATGCA^T
FauI 292 GCGGG CCCGC(4/6)
FbaI 1676 TGATCA T^GATCA
HaeII 2119 GGCGCC [GA]GCGC^[CT]
HhaI 2120 GCGC GCG^C
Hin1I 2119 GGCGCC G[GA]^CG[CT]C
HinP1I 2120 GCGC G^CGC
HpaI 519 GTTAAC GTT^AAC
KasI 2119 GGCGCC G^GCGCC
NarI 2119 GGCGCC GG^CGCC
NsiI 1247 ATGCAT ATGCA^T
PflMI 666 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1201 CGATCG CGAT^CG
RcaI 2213 TCATGA T^CATGA
SapI 2313 GCTCTTC GCTCTTC(1/4)
SfoI 2119 GGCGCC GGC^GCC
SspBI 1760 TGTACA T^GTACA
SwaI 719 ATTTAAAT ATTT^AAAT
Van91I 666 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1387 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 385 GTAC GT^AC
1761 GTAC
AlwI 1751 GATCC GGATC(4/5)
2487 GGATC
AspHI 22 GTGCTC G[AT]GC[AT]^C
1010 GAGCAC
AvaII 1281 GGACC G^G[AT]CC
1694 GGACC
BbsI 1591 GAAGAC GAAGAC(2/6)
196 GTCTTC
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
90 GCTGAGG
BcnI 11 CCCGG CC^[GC]GG
59 CCGGG
BglI 1831 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2065 GCCCTGCTGGC
BpuAI 1591 GAAGAC GAAGAC(2/6)
196 GTCTTC
BseRI 2361 CTCCTC GAGGAG(10/8)
277 GAGGAG
BsiEI 56 CGACCG CG[GA][CT]^CG
1201 CGATCG
BsiHKAI 22 GTGCTC G[AT]GC[AT]^C
1010 GAGCAC
BspMI 1658 ACCTGC ACCTGC(4/8)
2636 ACCTGC
DsaI 2081 CCATGG C^C[GA][CT]GG
2165 CCATGG
EarI 1025 CTCTTC CTCTTC(1/4)
2314 CTCTTC
HapII 12 CCGG C^CGG
59 CCGG
HgaI 194 GCGTC GACGC(5/10)
429 GCGTC
HincII 519 GTTAAC GT[CT]^[GA]AC
1171 GTTGAC
HindII 519 GTTAAC GT[CT]^[GA]AC
1171 GTTGAC
HindIII 1123 AAGCTT A^AGCTT
2541 AAGCTT
HpaII 12 CCGG C^CGG
59 CCGG
Ksp632I 1025 CTCTTC CTCTTC(1/4)
2314 CTCTTC
MslI 859 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1240 CACCCCAATG
MspA1I 49 CAGCTG C[AC]G^C[GT]G
238 CAGCTG
MspI 12 CCGG C^CGG
59 CCGG
NciI 11 CCCGG CC^[GC]GG
59 CCGGG
NcoI 2081 CCATGG C^CATGG
2165 CCATGG
NspI 1808 ACATGC [GA]CATG^[CT]
2437 GCATGT
PvuII 49 CAGCTG CAG^CTG
238 CAGCTG
RsaI 385 GTAC GT^AC
1761 GTAC
SspI 2000 AATATT AAT^ATT
2272 AATATT
TaqI 811 TCGA T^CGA
2570 TCGA
TthHB8I 811 TCGA T^CGA
2570 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI ApoI BanI
BanII BbvI BfaI BglII BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII