Restriction map for
pFN21AE2736
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeIII HapII HgaI Hin1I
HincII HindII HpaI HpaII HphI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MnlI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI StuI SwaI TaqI
Tsp45I TspRI Tth111I TthHB8I Van91I
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 612 AAATTT [GA]^AATT[CT]
AfaI 97 GTAC GT^AC
ApoI 612 AAATTT [GA]^AATT[CT]
AvaII 684 GGTCC G^G[AT]CC
AvrII 312 CCTAGG C^CTAGG
BanI 187 GGTGCC G^G[CT][GA]CC
BciVI 308 GTATCC GTATCC(6/5)
BlnI 312 CCTAGG C^CTAGG
BsaJI 312 CCTAGG C^C[ACGT][ACGT]GG
BsiYI 291 CCTGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 291 CCTGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 197 TCATGA T^CATGA
BsrDI 107 GCAATG GCAATG(2/0)
CfoI 386 GCGC GCG^C
Cfr13I 684 GGTCC G^G[ACGT]CC
DdeI 332 CTAAG C^T[ACGT]AG
DraI 445 TTTAAA TTT^AAA
EarI 439 GAAGAG CTCTTC(1/4)
EcoT14I 312 CCTAGG C^C[AT][AT]GG
HaeII 385 AGCGCC [GA]GCGC^[CT]
HhaI 386 GCGC GCG^C
HinP1I 386 GCGC G^CGC
HindIII 376 AAGCTT A^AGCTT
Ksp632I 439 GAAGAG CTCTTC(1/4)
MaeII 158 ACGT A^CGT
MseI 446 TTAA T^TAA
MwoI 181 GCTGTAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 187 GGTGCC GG[ACGT]^[ACGT]CC
PleI 112 GACTC GAGTC(4/5)
PstI 292 CTGCAG CTGCA^G
RcaI 197 TCATGA T^CATGA
RsaI 97 GTAC GT^AC
Sau96I 684 GGTCC G^G[ACGT]CC
SbfI 291 CCTGCAGG CCTGCA^GG
SmlI 116 CTCAAG C^T[CT][GA]AG
Sse8387I 291 CCTGCAGG CCTGCA^GG
SspI 26 AATATT AAT^ATT
StyI 312 CCTAGG C^C[AT][AT]GG
TfiI 201 GATTC G^A[AT]TC
Tru9I 446 TTAA T^TAA
XbaI 522 TCTAGA T^CTAGA
XcmI 543 CCAAAGCTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 224 GCGG CCGC(-3/-1)
455 GCGG
AlwI 75 GATCC GGATC(4/5)
282 GATCC
BsaI 534 GAGACC GGTCTC(1/5)
574 GAGACC
BsmAI 534 GAGAC GTCTC(1/5)
574 GAGAC
BspMI 4 GCAGGT ACCTGC(4/8)
294 GCAGGT
BsrI 645 ACTGG ACTGG(1/-1)
663 ACTGG
BssKI 213 CCTGG ^CC[ACGT]GG
414 CCAGG
BstNI 213 CCTGG CC^[AT]GG
414 CCAGG
EcoRII 213 CCTGG ^CC[AT]GG
414 CCAGG
HinfI 112 GACTC G^A[ACGT]TC
201 GATTC
MboII 136 GAAGA GAAGA(8/7)
439 GAAGA
MslI 192 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
423 CATAATTGTG
MvaI 213 CCTGG CC^[AT]GG
414 CCAGG
NspI 217 GCATGC [GA]CATG^[CT]
248 GCATGC
ScrFI 213 CCTGG CC^[ACGT]GG
414 CCAGG
SfcI 182 CTGTAG C^T[GA][CT]AG
292 CTGCAG
SphI 217 GCATGC GCATG^C
248 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI BbvI BfaI Cac8I DpnII
Fnu4HI ItaI MaeI MboI NdeII
NlaIII Sau3AI TseI Tsp509I