Restriction map for
pFN21AE1661
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MaeIII MamI MflI
MluI MroI MseI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 82 GTAC GT^AC
AflIII 391 ACATGT A^C[GA][CT]GT
AlwNI 280 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 260 AGCGCT AGC^GCT
Asp700I 231 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 24 GAGCTC G[AT]GC[AT]^C
AvaII 41 GGACC G^G[AT]CC
BanI 354 GGTGCC G^G[CT][GA]CC
BanII 24 GAGCTC G[GA]GC[CT]^C
BfaI 188 CTAG C^TAG
BseRI 89 GAGGAG GAGGAG(10/8)
BsiHKAI 24 GAGCTC G[AT]GC[AT]^C
BsmBI 217 CGTCTC CGTCTC(1/5)
BsmFI 40 GGGAC GGGAC(10/14)
BspLU11I 391 ACATGT A^CATGT
BsrI 498 ACTGG ACTGG(1/-1)
DpnII 480 GATC ^GATC
DraII 40 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 240 CCACGG C^C[GA][CT]GG
Eco47III 260 AGCGCT AGC^GCT
EcoO109I 40 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 260 AGCGCT [GA]GCGC^[CT]
HindIII 460 AAGCTT A^AGCTT
HinfI 245 GAGTC G^A[ACGT]TC
MaeI 188 CTAG C^TAG
MaeII 212 ACGT A^CGT
MboI 480 GATC ^GATC
MboII 301 GAAGA GAAGA(8/7)
MfeI 488 CAATTG C^AATTG
MslI 165 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 488 CAATTG C^AATTG
NdeII 480 GATC ^GATC
NheI 187 GCTAGC G^CTAGC
NspI 391 ACATGT [GA]CATG^[CT]
PleI 245 GAGTC GAGTC(4/5)
PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 211 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 82 GTAC GT^AC
SacI 24 GAGCTC GAGCT^C
Sau3AI 480 GATC ^GATC
Tsp509I 489 AATT ^AATT
XmnI 231 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 5 CCGC CCGC(-3/-1)
162 CCGC
BalI 47 TGGCCA TGG^CCA
365 TGGCCA
BlpI 372 GCTCAGC GC^T[ACGT]AGC
384 GCTAAGC
BmyI 24 GAGCTC G[AGT]GC[ACT]^C
355 GTGCCC
Bpu1102I 372 GCTCAGC GC^T[ACGT]AGC
384 GCTAAGC
BsaJI 44 CCCTGG C^C[ACGT][ACGT]GG
240 CCACGG
BsiYI 63 CCTTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCCACTGCAGG
BslI 63 CCTTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCCACTGCAGG
BsmAI 218 GTCTC GTCTC(1/5)
516 GTCTC
Bsp1286I 24 GAGCTC G[AGT]GC[ACT]^C
355 GTGCCC
BspMI 413 ACCTGC ACCTGC(4/8)
279 GCAGGT
CelII 372 GCTCAGC GC^T[ACGT]AGC
384 GCTAAGC
CfoI 100 GCGC GCG^C
261 GCGC
Cfr13I 41 GGACC G^G[ACGT]CC
72 GGCCC
HhaI 100 GCGC GCG^C
261 GCGC
HinP1I 100 GCGC G^CGC
261 GCGC
MluNI 47 TGGCCA TGG^CCA
365 TGGCCA
MscI 47 TGGCCA TGG^CCA
365 TGGCCA
MspA1I 54 CAGCTG C[AC]G^C[GT]G
95 CAGCTG
NlaIII 171 CATG CATG^
392 CATG
PstI 10 CTGCAG CTGCA^G
117 CTGCAG
PvuII 54 CAGCTG CAG^CTG
95 CAGCTG
Sau96I 41 GGACC G^G[ACGT]CC
72 GGCCC
SfcI 10 CTGCAG C^T[GA][CT]AG
117 CTGCAG
TspRI 115 CACTG CA[GC]TG[ACGT][ACGT]^
452 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BssKI BstNI Cac8I
DdeI EaeI EcoRII Fnu4HI HaeIII
HphI ItaI MnlI MvaI MwoI
NlaIV ScrFI TseI