##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TfiI        TspRI       Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI          136  GCGG                 CCGC(-3/-1)
AflII         100  CTTAAG               C^TTAAG
AlwNI         466  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII          72  GGACC                G^G[AT]CC
BbsI          292  GAAGAC               GAAGAC(2/6)
BbvI          436  GCAGC                GCAGC(8/12)
BfrI          100  CTTAAG               C^TTAAG
BpuAI         292  GAAGAC               GAAGAC(2/6)
BsaI          280  GAGACC               GGTCTC(1/5)
BseRI         181  GAGGAG               GAGGAG(10/8)
BsiYI         411  CCAAGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          411  CCAAGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         118  GCAGGT               ACCTGC(4/8)
BssKI          69  CCAGG                ^CC[ACGT]GG
BstBI         343  TTCGAA               TT^CGAA
BstNI          69  CCAGG                CC^[AT]GG
Cac8I         432  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr13I         72  GGACC                G^G[ACGT]CC
DdeI          156  CTTAG                C^T[ACGT]AG
DraII          71  AGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          485  GAAGAG               CTCTTC(1/4)
EcoO109I       71  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII         69  CCAGG                ^CC[AT]GG
Fnu4HI        436  GCAGC                GC^[ACGT]GC
HinfI         462  GAGTC                G^A[ACGT]TC
ItaI          436  GCAGC                GC^[ACGT]GC
Ksp632I       485  GAAGAG               CTCTTC(1/4)
MvaI           69  CCAGG                CC^[AT]GG
MwoI           59  GCTGTTACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI           77  CATATG               CA^TATG
NlaIV          72  GGACCC               GG[ACGT]^[ACGT]CC
NspV          343  TTCGAA               TT^CGAA
PflMI         411  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          462  GAGTC                GAGTC(4/5)
PpuMI          71  AGGACCC              [GA]G^G[AT]CC[CT]
SapI          485  GAAGAGC              GCTCTTC(1/4)
Sau96I         72  GGACC                G^G[ACGT]CC
ScrFI          69  CCAGG                CC^[ACGT]GG
SfuI          343  TTCGAA               TT^CGAA
SmlI          100  CTTAAG               C^T[CT][GA]AG
SspI          107  AATATT               AAT^ATT
TseI          436  GCAGC                G^C[AT]GC
Tsp45I        188  GTGAC                ^GT[GC]AC
Van91I        411  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BsmAI         280  GAGAC                GTCTC(1/5)
              217  GTCTC               
DraI          238  TTTAAA               TTT^AAA
              247  TTTAAA              
DrdI          295  GACATGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              373  GACTTGAATGTC        
MnlI          181  GAGG                 CCTC(7/6)
              479  GAGG                
MslI          124  CATTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              408  CATCCAAGTG          
NlaIII        297  CATG                 CATG^
              400  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BstF5I      FokI        MaeIII      MboII       
MseI        SfaNI       TaqI        Tru9I       Tsp509I     
TthHB8I