Restriction map for
pFN21AB6149
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI CelII ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PstI PvuI RcaI
RsrII SalI SbfI SexAI SfaNI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 847 AACGTT AA^CGTT
AflII 1659 CTTAAG C^TTAAG
AlwNI 695 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1485 CCTGAGG CC^T[ACGT]AGG
ApaI 17 GGGCCC GGGCC^C
AseI 810 ATTAAT AT^TAAT
AsnI 810 ATTAAT AT^TAAT
AspHI 79 GAGCTC G[AT]GC[AT]^C
AspI 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 906 TGATCA T^GATCA
BfrI 1659 CTTAAG C^TTAAG
BsaWI 950 TCCGGT [AT]^CCGG[AT]
BseRI 1400 CTCCTC GAGGAG(10/8)
BsgI 841 CTGCAC GTGCAG(16/14)
BsiHKAI 79 GAGCTC G[AT]GC[AT]^C
BsrFI 1268 GCCGGT [GA]^CCGG[CT]
Bst1107I 535 GTATAC GTA^TAC
BstAPI 39 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 797 TTCGAA TT^CGAA
BstEII 250 GGTGACC G^GT[ACGT]ACC
BstPI 250 GGTGACC G^GT[ACGT]ACC
BstXI 781 CCACTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 535 GTATAC GTA^TAC
Bsu36I 1485 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1268 GCCGGT [GA]^CCGG[CT]
Eco81I 1485 CCTGAGG CC^T[ACGT]AGG
EcoNI 255 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 250 GGTGACC G^GT[ACGT]ACC
FbaI 906 TGATCA T^GATCA
HindIII 1589 AAGCTT A^AGCTT
KspI 21 CCGCGG CCGC^GG
MaeII 848 ACGT A^CGT
MslI 981 CATAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 938 CCATGG C^CATGG
NdeI 563 CATATG CA^TATG
NspV 797 TTCGAA TT^CGAA
PflFI 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 270 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 810 ATTAAT AT^TAAT
Psp1406I 847 AACGTT AA^CGTT
SacI 79 GAGCTC GAGCT^C
SacII 21 CCGCGG CCGC^GG
SapI 1251 GCTCTTC GCTCTTC(1/4)
SfcI 1071 CTACAG C^T[GA][CT]AG
SfuI 797 TTCGAA TT^CGAA
Tth111I 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 270 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1206 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 17 GGGCCC G[GA]GC[CT]^C
79 GAGCTC
BsmFI 279 GGGAC GGGAC(10/14)
954 GTCCC
BsmI 1522 GAATGC GAATGC(1/-1)
1707 GCATTC
BspMI 56 ACCTGC ACCTGC(4/8)
839 ACCTGC
BssHII 26 GCGCGC G^CGCGC
110 GCGCGC
DraI 580 TTTAAA TTT^AAA
1317 TTTAAA
HapII 951 CCGG C^CGG
1269 CCGG
HpaII 951 CCGG C^CGG
1269 CCGG
MspI 951 CCGG C^CGG
1269 CCGG
NspI 1019 ACATGC [GA]CATG^[CT]
1598 ACATGC
PleI 1503 GACTC GAGTC(4/5)
327 GAGTC
ScaI 438 AGTACT AGT^ACT
1333 AGTACT
SmlI 1337 CTCAAG C^T[CT][GA]AG
1659 CTTAAG
StuI 1182 AGGCCT AGG^CCT
1482 AGGCCT
XcmI 189 CCAAGGTAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
389 CCAATGTCACCGTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII