Restriction map for
pFN21AB8542
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI BanI BbeI BbrPI
BglI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1792 CGCG CG^CG
AcsI 1777 AAATTC [GA]^AATT[CT]
AcyI 22 GGCGTC G[GA]^CG[CT]C
AflII 1288 CTTAAG C^TTAAG
ApoI 1777 AAATTC [GA]^AATT[CT]
Asp700I 331 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2114 TGCGCA TGC^GCA
AvrII 1704 CCTAGG C^CTAGG
BalI 1655 TGGCCA TGG^CCA
BbsI 1531 GAAGAC GAAGAC(2/6)
BclI 2031 TGATCA T^GATCA
BfrI 1288 CTTAAG C^TTAAG
BglII 992 AGATCT A^GATCT
BlnI 1704 CCTAGG C^CTAGG
BlpI 1697 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1697 GCTCAGC GC^T[ACGT]AGC
BpuAI 1531 GAAGAC GAAGAC(2/6)
BsaHI 22 GGCGTC G[GA]^CG[CT]C
Bsp1407I 1865 TGTACA T^GTACA
BsrFI 39 GCCGGT [GA]^CCGG[CT]
BsrGI 1865 TGTACA T^GTACA
BstEII 1243 GGTGACC G^GT[ACGT]ACC
BstPI 1243 GGTGACC G^GT[ACGT]ACC
BstUI 1792 CGCG CG^CG
BstXI 959 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1697 GCTCAGC GC^T[ACGT]AGC
Cfr10I 39 GCCGGT [GA]^CCGG[CT]
EarI 175 CTCTTC CTCTTC(1/4)
EcoO65I 1243 GGTGACC G^GT[ACGT]ACC
FbaI 2031 TGATCA T^GATCA
FspI 2114 TGCGCA TGC^GCA
HgaI 23 GCGTC GACGC(5/10)
Hin1I 22 GGCGTC G[GA]^CG[CT]C
HincII 823 GTCAAC GT[CT]^[GA]AC
HindII 823 GTCAAC GT[CT]^[GA]AC
Ksp632I 175 CTCTTC CTCTTC(1/4)
KspI 1791 CCGCGG CCGC^GG
MluNI 1655 TGGCCA TGG^CCA
MscI 1655 TGGCCA TGG^CCA
MvnI 1792 CGCG CG^CG
NspI 2276 ACATGC [GA]CATG^[CT]
PpuMI 140 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 2062 GAGCTC GAGCT^C
SacII 1791 CCGCGG CCGC^GG
SpeI 184 ACTAGT A^CTAGT
SspBI 1865 TGTACA T^GTACA
XmnI 331 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 2444 AGCGCT AGC^GCT
2529 AGCGCT
BamHI 1832 GGATCC G^GATCC
2099 GGATCC
BciVI 2120 GTATCC GTATCC(6/5)
2336 GTATCC
BsaI 955 GAGACC GGTCTC(1/5)
1490 GAGACC
BsgI 505 CTGCAC GTGCAG(16/14)
1330 GTGCAG
BspMI 1358 ACCTGC ACCTGC(4/8)
1910 ACCTGC
BsrDI 1403 CATTGC GCAATG(2/0)
1743 CATTGC
DraII 140 AGGTCCC [GA]G^G[ACGT]CC[CT]
725 AGGGCCT
DsaI 1791 CCGCGG C^C[GA][CT]GG
2282 CCACGG
EaeI 47 TGGCCG [CT]^GGCC[GA]
1655 TGGCCA
Eco47III 2444 AGCGCT AGC^GCT
2529 AGCGCT
EcoNI 483 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
681 CCTATCTGAGG
EcoO109I 140 AGGTCCC [GA]G^G[ACGT]CC[CT]
725 AGGGCCT
MaeII 821 ACGT A^CGT
2470 ACGT
PflMI 246 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2406 CCAAAAATTGG
PleI 363 GACTC GAGTC(4/5)
384 GAGTC
SfaNI 1558 GATGC GCATC(5/9)
2107 GATGC
SmaI 27 CCCGGG CCC^GGG
65 CCCGGG
TfiI 421 GAATC G^A[AT]TC
762 GAATC
Van91I 246 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2406 CCAAAAATTGG
XmaCI 27 CCCGGG C^CCGGG
65 CCCGGG
XmaI 27 CCCGGG C^CCGGG
65 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanII
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII