Restriction map for
pFN21AE1834
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 743 GTCGAC GT^[AC][GT]AC
AcyI 965 GGCGCC G[GA]^CG[CT]C
AlwI 791 GGATC GGATC(4/5)
AocI 802 CCTGAGG CC^T[ACGT]AGG
ApaI 91 GGGCCC GGGCC^C
AvaI 668 CCCGGG C^[CT]CG[GA]G
AvrII 543 CCTAGG C^CTAGG
BbeI 965 GGCGCC GGCGC^C
BlnI 543 CCTAGG C^CTAGG
BsaHI 965 GGCGCC G[GA]^CG[CT]C
BsoBI 668 CCCGGG C^[CT]CG[GA]G
BspMI 503 ACCTGC ACCTGC(4/8)
BsrDI 11 CATTGC GCAATG(2/0)
BstAPI 710 GCATCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 802 CCTGAGG CC^T[ACGT]AGG
DrdI 376 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 802 CCTGAGG CC^T[ACGT]AGG
EcoNI 504 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 117 GCGGG CCCGC(4/6)
Hin1I 965 GGCGCC G[GA]^CG[CT]C
HincII 743 GTCGAC GT[CT]^[GA]AC
HindII 743 GTCGAC GT[CT]^[GA]AC
KasI 965 GGCGCC G^GCGCC
MaeIII 374 GTGAC ^GT[ACGT]AC
MboII 900 TCTTC GAAGA(8/7)
MslI 885 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 965 GGCGCC GG^CGCC
PpuMI 242 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 917 CAGCTG CAG^CTG
SacI 621 GAGCTC GAGCT^C
SalI 743 GTCGAC G^TCGAC
SfoI 965 GGCGCC GGC^GCC
SmaI 668 CCCGGG CCC^GGG
SphI 857 GCATGC GCATG^C
TfiI 696 GATTC G^A[AT]TC
Tsp45I 374 GTGAC ^GT[GC]AC
XbaI 261 TCTAGA T^CTAGA
XmaCI 668 CCCGGG C^CCGGG
XmaI 668 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AlwNI 499 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
522 CAGTGGCTG
AspHI 621 GAGCTC G[AT]GC[AT]^C
892 GTGCTC
BalI 50 TGGCCA TGG^CCA
137 TGGCCA
BfaI 262 CTAG C^TAG
544 CTAG
BsiHKAI 621 GAGCTC G[AT]GC[AT]^C
892 GTGCTC
BsmFI 995 GGGAC GGGAC(10/14)
244 GTCCC
CfoI 177 GCGC GCG^C
966 GCGC
DpnII 133 GATC ^GATC
792 GATC
DsaI 237 CCACGG C^C[GA][CT]GG
956 CCACGG
EaeI 50 TGGCCA [CT]^GGCC[GA]
137 TGGCCA
HaeII 176 AGCGCC [GA]GCGC^[CT]
965 GGCGCC
HapII 669 CCGG C^CGG
963 CCGG
HhaI 177 GCGC GCG^C
966 GCGC
HinP1I 177 GCGC G^CGC
966 GCGC
HpaII 669 CCGG C^CGG
963 CCGG
MaeI 262 CTAG C^TAG
544 CTAG
MboI 133 GATC ^GATC
792 GATC
MluNI 50 TGGCCA TGG^CCA
137 TGGCCA
MscI 50 TGGCCA TGG^CCA
137 TGGCCA
MspA1I 115 CAGCGG C[AC]G^C[GT]G
917 CAGCTG
MspI 669 CCGG C^CGG
963 CCGG
NdeII 133 GATC ^GATC
792 GATC
NspI 82 GCATGT [GA]CATG^[CT]
857 GCATGC
PflMI 79 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
521 CCAGTGGCTGG
PleI 333 GACTC GAGTC(4/5)
452 GAGTC
Sau3AI 133 GATC ^GATC
792 GATC
SmlI 557 CTCAAG C^T[CT][GA]AG
904 CTCAAG
TaqI 713 TCGA T^CGA
744 TCGA
TthHB8I 713 TCGA T^CGA
744 TCGA
Van91I 79 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
521 CCAGTGGCTGG
XcmI 95 CCACCTTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAACCTCACCTTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MnlI
MvaI MwoI NciI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfaNI
SfcI StuI StyI TseI TspRI