Restriction map for
pFN21AE0704
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BcgI
BclI BfrI BglI BlnI BmyI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 733 GGCGTC G[GA]^CG[CT]C
AlwI 631 GATCC GGATC(4/5)
AvaI 25 CCCGGG C^[CT]CG[GA]G
BanI 93 GGCACC G^G[CT][GA]CC
BbsI 861 GTCTTC GAAGAC(2/6)
BbvCI 1044 CCTCAGC CCTCAGC(-5/-2)
BciVI 494 GTATCC GTATCC(6/5)
BfaI 101 CTAG C^TAG
BglII 210 AGATCT A^GATCT
BpuAI 861 GTCTTC GAAGAC(2/6)
BsaAI 1059 CACGTA [CT]AC^GT[GA]
BsaHI 733 GGCGTC G[GA]^CG[CT]C
BsgI 830 CTGCAC GTGCAG(16/14)
BsmI 720 GAATGC GAATGC(1/-1)
BsoBI 25 CCCGGG C^[CT]CG[GA]G
Bsp1407I 363 TGTACA T^GTACA
BsrDI 140 GCAATG GCAATG(2/0)
BsrGI 363 TGTACA T^GTACA
BstXI 579 CCACTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 210 AGATCT [GA]^GATC[CT]
DrdI 727 GACATGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 767 CTCTTC CTCTTC(1/4)
FauI 659 GCGGG CCCGC(4/6)
HapII 26 CCGG C^CGG
Hin1I 733 GGCGTC G[GA]^CG[CT]C
HpaII 26 CCGG C^CGG
Ksp632I 767 CTCTTC CTCTTC(1/4)
MaeI 101 CTAG C^TAG
MaeII 1060 ACGT A^CGT
MflI 210 AGATCT [GA]^GATC[CT]
MspI 26 CCGG C^CGG
PflMI 1023 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1076 CTGCAG CTGCA^G
PvuII 154 CAGCTG CAG^CTG
SapI 766 GCTCTTC GCTCTTC(1/4)
SfcI 1076 CTGCAG C^T[GA][CT]AG
SmaI 25 CCCGGG CCC^GGG
SspBI 363 TGTACA T^GTACA
Van91I 1023 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 210 AGATCT [GA]^GATC[CT]
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 445 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1024 CAGCAACTG
BalI 474 TGGCCA TGG^CCA
712 TGGCCA
BcnI 25 CCCGG CC^[GC]GG
26 CCGGG
BlpI 82 GCTCAGC GC^T[ACGT]AGC
156 GCTGAGC
Bpu1102I 82 GCTCAGC GC^T[ACGT]AGC
156 GCTGAGC
BseRI 890 CTCCTC GAGGAG(10/8)
1141 GAGGAG
BsmFI 514 GGGAC GGGAC(10/14)
1011 GGGAC
CelII 82 GCTCAGC GC^T[ACGT]AGC
156 GCTGAGC
DpnII 211 GATC ^GATC
631 GATC
DraII 395 AGGACCC [GA]G^G[ACGT]CC[CT]
1011 GGGACCC
EcoO109I 395 AGGACCC [GA]G^G[ACGT]CC[CT]
1011 GGGACCC
EcoT14I 681 CCAAGG C^C[AT][AT]GG
715 CCAAGG
HgaI 1177 GACGC GACGC(5/10)
734 GCGTC
HinfI 547 GAGTC G^A[ACGT]TC
879 GACTC
HphI 575 TCACC GGTGA(8/7)
803 TCACC
MboI 211 GATC ^GATC
631 GATC
MluNI 474 TGGCCA TGG^CCA
712 TGGCCA
MscI 474 TGGCCA TGG^CCA
712 TGGCCA
MseI 584 TTAA T^TAA
599 TTAA
NciI 25 CCCGG CC^[GC]GG
26 CCGGG
NdeI 68 CATATG CA^TATG
233 CATATG
NdeII 211 GATC ^GATC
631 GATC
PleI 879 GACTC GAGTC(4/5)
547 GAGTC
PpuMI 395 AGGACCC [GA]G^G[AT]CC[CT]
1011 GGGACCC
Sau3AI 211 GATC ^GATC
631 GATC
StyI 681 CCAAGG C^C[AT][AT]GG
715 CCAAGG
Tru9I 584 TTAA T^TAA
599 TTAA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BpmI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MboII MnlI MslI MspA1I MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I