Restriction map for
pFN21AE3083
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MscI MseI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 479 TCCGGA T^CCGGA
AfaI 487 GTAC GT^AC
AlwI 630 GGATC GGATC(4/5)
ApaI 703 GGGCCC GGGCC^C
BanI 344 GGCACC G^G[CT][GA]CC
BfaI 289 CTAG C^TAG
BseAI 479 TCCGGA T^CCGGA
BsmFI 13 GTCCC GGGAC(10/14)
BspEI 479 TCCGGA T^CCGGA
BspMI 283 ACCTGC ACCTGC(4/8)
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BstEII 279 GGTCACC G^GT[ACGT]ACC
BstPI 279 GGTCACC G^GT[ACGT]ACC
BstXI 410 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 630 GGATCT [GA]^GATC[CT]
DpnII 631 GATC ^GATC
DrdI 4 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 410 CCATGG C^C[GA][CT]GG
EaeI 119 TGGCCG [CT]^GGCC[GA]
EarI 172 CTCTTC CTCTTC(1/4)
EcoNI 468 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 279 GGTCACC G^GT[ACGT]ACC
HinfI 129 GACTC G^A[ACGT]TC
Ksp632I 172 CTCTTC CTCTTC(1/4)
MaeI 289 CTAG C^TAG
MaeIII 280 GTCAC ^GT[ACGT]AC
MboI 631 GATC ^GATC
MboII 173 TCTTC GAAGA(8/7)
MflI 630 GGATCT [GA]^GATC[CT]
MroI 479 TCCGGA T^CCGGA
NcoI 410 CCATGG C^CATGG
NdeI 536 CATATG CA^TATG
NdeII 631 GATC ^GATC
NspI 522 GCATGT [GA]CATG^[CT]
PflMI 708 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 129 GACTC GAGTC(4/5)
PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 487 GTAC GT^AC
SacI 169 GAGCTC GAGCT^C
SapI 171 GCTCTTC GCTCTTC(1/4)
Sau3AI 631 GATC ^GATC
SbfI 468 CCTGCAGG CCTGCA^GG
ScaI 486 AGTACT AGT^ACT
SmlI 634 CTTGAG C^T[CT][GA]AG
Sse8387I 468 CCTGCAGG CCTGCA^GG
TaqI 572 TCGA T^CGA
Tsp45I 280 GTCAC ^GT[GC]AC
TthHB8I 572 TCGA T^CGA
Van91I 708 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 630 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 25 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
85 CAGCCCCTG
BpmI 223 CTGGAG CTGGAG(16/14)
377 CTGGAG
BsaI 492 GAGACC GGTCTC(1/5)
619 GAGACC
BsaJI 410 CCATGG C^C[ACGT][ACGT]GG
457 CCAAGG
BsaWI 241 ACCGGA [AT]^CCGG[AT]
479 TCCGGA
BsrI 222 ACTGG ACTGG(1/-1)
645 CCAGT
Cac8I 80 GCTGGC GC[ACGT]^[ACGT]GC
396 GCTGGC
DdeI 335 CTGAG C^T[ACGT]AG
490 CTGAG
DraII 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
703 GGGCCCC
EcoO109I 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
703 GGGCCCC
EcoT14I 410 CCATGG C^C[AT][AT]GG
457 CCAAGG
HphI 281 TCACC GGTGA(8/7)
611 TCACC
MslI 411 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
429 CACTCTGGTG
MspA1I 25 CAGCTG C[AC]G^C[GT]G
394 CAGCTG
MwoI 27 GCTGCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
30 GCTGGTGCTGC
PvuII 25 CAGCTG CAG^CTG
394 CAGCTG
StyI 410 CCATGG C^C[AT][AT]GG
457 CCAAGG
XcmI 410 CCATGGATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
535 CCATATGGAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BmyI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cfr13I EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
MnlI MspI MvaI NciI NlaIII
NlaIV PstI Sau96I ScrFI SfcI
TseI