Restriction map for
pFN21AE2118
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
BalI BbeI BbrPI BciVI BclI
BfrI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoT22I
FbaI FseI HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcyI 126 GACGCC G[GA]^CG[CT]C
AflIII 2369 ACATGT A^C[GA][CT]GT
Alw44I 537 GTGCAC G^TGCAC
ApaLI 537 GTGCAC G^TGCAC
AviII 77 TGCGCA TGC^GCA
AvrII 1737 CCTAGG C^CTAGG
BamHI 1890 GGATCC G^GATCC
BanI 1400 GGTGCC G^G[CT][GA]CC
BcgI 232 CGACTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 752 AGATCT A^GATCT
BlnI 1737 CCTAGG C^CTAGG
BlpI 1940 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1940 GCTCAGC GC^T[ACGT]AGC
BsaHI 126 GACGCC G[GA]^CG[CT]C
Bsp1407I 2111 TGTACA T^GTACA
BspHI 1794 TCATGA T^CATGA
BspLU11I 2369 ACATGT A^CATGT
BsrGI 2111 TGTACA T^GTACA
BstEII 188 GGTCACC G^GT[ACGT]ACC
BstPI 188 GGTCACC G^GT[ACGT]ACC
BstXI 1704 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1940 GCTCAGC GC^T[ACGT]AGC
DraIII 1928 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 188 GGTCACC G^GT[ACGT]ACC
EcoRV 1519 GATATC GAT^ATC
FspI 77 TGCGCA TGC^GCA
HgaI 126 GACGC GACGC(5/10)
Hin1I 126 GACGCC G[GA]^CG[CT]C
NcoI 650 CCATGG C^CATGG
RcaI 1794 TCATGA T^CATGA
SacI 1252 GAGCTC GAGCT^C
SalI 871 GTCGAC G^TCGAC
SspBI 2111 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AhdI 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1834 GACCTGCTGTC
AspEI 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1834 GACCTGCTGTC
AspI 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1613 GACGGAGTC
BsaI 16 GAGACC GGTCTC(1/5)
464 GAGACC
BsrDI 1095 CATTGC GCAATG(2/0)
848 GCAATG
EaeI 612 TGGCCG [CT]^GGCC[GA]
2347 CGGCCA
Eam1105I 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1834 GACCTGCTGTC
HaeII 425 GGCGCT [GA]GCGC^[CT]
1651 AGCGCC
HincII 640 GTTGAC GT[CT]^[GA]AC
871 GTCGAC
HindII 640 GTTGAC GT[CT]^[GA]AC
871 GTCGAC
HindIII 1385 AAGCTT A^AGCTT
1462 AAGCTT
MaeII 935 ACGT A^CGT
2142 ACGT
MslI 561 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
795 CACCAAGATG
PflFI 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1613 GACGGAGTC
PflMI 950 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2266 CCATCAGATGG
StuI 1407 AGGCCT AGG^CCT
1763 AGGCCT
TaqI 216 TCGA T^CGA
872 TCGA
Tsp509I 2491 AATT ^AATT
2522 AATT
Tth111I 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1613 GACGGAGTC
TthHB8I 216 TCGA T^CGA
872 TCGA
Van91I 950 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2266 CCATCAGATGG
XcmI 74 CCATGCGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
243 CCAGAGTGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI Tru9I
TseI Tsp45I TspRI XhoII XmaCI
XmaI