Restriction map for
pFN21AB5989
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcyI 1385 GGCGTC G[GA]^CG[CT]C
Aor51HI 953 AGCGCT AGC^GCT
ApaI 820 GGGCCC GGGCC^C
AvaI 287 CCCGAG C^[CT]CG[GA]G
BalI 966 TGGCCA TGG^CCA
BbrPI 1565 CACGTG CAC^GTG
BglII 996 AGATCT A^GATCT
BsaAI 1565 CACGTG [CT]AC^GT[GA]
BsaHI 1385 GGCGTC G[GA]^CG[CT]C
BsaI 1049 GAGACC GGTCTC(1/5)
BsgI 1372 GTGCAG GTGCAG(16/14)
BsoBI 287 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1500 TGTACA T^GTACA
BspMI 322 ACCTGC ACCTGC(4/8)
BsrDI 347 GCAATG GCAATG(2/0)
BsrGI 1500 TGTACA T^GTACA
BstAPI 591 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 763 GACCACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 316 CTCTTC CTCTTC(1/4)
Eco47III 953 AGCGCT AGC^GCT
FauI 96 GCGGG CCCGC(4/6)
HgaI 1386 GCGTC GACGC(5/10)
Hin1I 1385 GGCGTC G[GA]^CG[CT]C
Ksp632I 316 CTCTTC CTCTTC(1/4)
MaeII 1566 ACGT A^CGT
MluNI 966 TGGCCA TGG^CCA
MscI 966 TGGCCA TGG^CCA
PmaCI 1565 CACGTG CAC^GTG
PmlI 1565 CACGTG CAC^GTG
PstI 589 CTGCAG CTGCA^G
SexAI 978 ACCAGGT A^CC[AT]GGT
SmlI 1532 CTCAAG C^T[CT][GA]AG
SspBI 1500 TGTACA T^GTACA
TfiI 1686 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 188 GGATC GGATC(4/5)
1033 GGATC
BanI 1144 GGCACC G^G[CT][GA]CC
1161 GGCACC
BbsI 1453 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BglI 682 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1315 GCCTATGAGGC
BpuAI 1453 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BsmAI 239 GAGAC GTCTC(1/5)
1049 GAGAC
BstYI 188 GGATCT [GA]^GATC[CT]
996 AGATCT
EaeI 804 TGGCCG [CT]^GGCC[GA]
966 TGGCCA
EcoNI 370 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
921 CCTGGCCCAGG
MaeIII 275 GTGAC ^GT[ACGT]AC
887 GTGAC
MflI 188 GGATCT [GA]^GATC[CT]
996 AGATCT
MslI 36 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CATCTGGATG
PflMI 884 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCAGGGCCTGG
PpuMI 405 GGGTCCC [GA]G^G[AT]CC[CT]
798 GGGTCCT
PshAI 277 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1288 GACAGCAGTC
Tsp45I 275 GTGAC ^GT[GC]AC
887 GTGAC
Tsp509I 355 AATT ^AATT
1605 AATT
Van91I 884 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCAGGGCCTGG
XcmI 294 CCACCCCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1043 CCATTGGAGACCTGG
XhoII 188 GGATCT [GA]^GATC[CT]
996 AGATCT
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TseI TspRI