Restriction map for
pFN21AE3233
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII BstBI
BstEII BstPI BstUI BstXI Cfr10I
ClaI CpoI DraI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeII
MamI MfeI MluI MluNI MscI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 217 GTATAC GT^[AC][GT]AC
AccIII 794 TCCGGA T^CCGGA
AcsI 775 GAATTT [GA]^AATT[CT]
AcyI 233 GGCGCC G[GA]^CG[CT]C
AlwNI 526 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1471 CCTGAGG CC^T[ACGT]AGG
ApoI 775 GAATTT [GA]^AATT[CT]
Asp700I 1347 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 233 GGCGCC G^G[CT][GA]CC
BbeI 233 GGCGCC GGCGC^C
BbvCI 558 GCTGAGG CCTCAGC(-5/-2)
BcgI 600 GCAAGAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 83 AGATCT A^GATCT
BsaHI 233 GGCGCC G[GA]^CG[CT]C
BsaWI 794 TCCGGA [AT]^CCGG[AT]
BseAI 794 TCCGGA T^CCGGA
BsgI 986 GTGCAG GTGCAG(16/14)
BsmI 7 GCATTC GAATGC(1/-1)
BspEI 794 TCCGGA T^CCGGA
BsrBI 1000 GAGCGG CCGCTC(-3/-3)
BsrDI 948 CATTGC GCAATG(2/0)
BssSI 1375 CTCGTG CACGAG(-5/-1)
Bst1107I 217 GTATAC GTA^TAC
BstAPI 525 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 217 GTATAC GTA^TAC
Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG
DraIII 317 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 426 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1471 CCTGAGG CC^T[ACGT]AGG
EcoT14I 237 CCAAGG C^C[AT][AT]GG
EcoT22I 5 ATGCAT ATGCA^T
Hin1I 233 GGCGCC G[GA]^CG[CT]C
KasI 233 GGCGCC G^GCGCC
MroI 794 TCCGGA T^CCGGA
MslI 813 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 233 GGCGCC GG^CGCC
NsiI 5 ATGCAT ATGCA^T
NspI 716 GCATGT [GA]CATG^[CT]
PaeR7I 1050 CTCGAG C^TCGAG
SapI 1105 GAAGAGC GCTCTTC(1/4)
SexAI 206 ACCAGGT A^CC[AT]GGT
SfoI 233 GGCGCC GGC^GCC
SmaI 740 CCCGGG CCC^GGG
StuI 1119 AGGCCT AGG^CCT
StyI 237 CCAAGG C^C[AT][AT]GG
Tsp509I 776 AATT ^AATT
XcmI 815 CCATTATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1050 CTCGAG C^TCGAG
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
XmnI 1347 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 551 GGGCCC GGGCC^C
1243 GGGCCC
AspHI 370 GAGCAC G[AT]GC[AT]^C
911 GAGCAC
BlpI 1147 GCTCAGC GC^T[ACGT]AGC
1316 GCTCAGC
Bpu1102I 1147 GCTCAGC GC^T[ACGT]AGC
1316 GCTCAGC
BsaI 925 GAGACC GGTCTC(1/5)
22 GGTCTC
BsiHKAI 370 GAGCAC G[AT]GC[AT]^C
911 GAGCAC
BspMI 977 ACCTGC ACCTGC(4/8)
846 GCAGGT
BstYI 83 AGATCT [GA]^GATC[CT]
1341 GGATCT
CelII 1147 GCTCAGC GC^T[ACGT]AGC
1316 GCTCAGC
CfoI 234 GCGC GCG^C
291 GCGC
DraII 551 GGGCCCT [GA]G^G[ACGT]CC[CT]
1243 GGGCCCC
EcoO109I 551 GGGCCCT [GA]G^G[ACGT]CC[CT]
1243 GGGCCCC
HaeII 233 GGCGCC [GA]GCGC^[CT]
290 AGCGCC
HgaI 456 GACGC GACGC(5/10)
767 GACGC
HhaI 234 GCGC GCG^C
291 GCGC
HinP1I 234 GCGC G^CGC
291 GCGC
MflI 83 AGATCT [GA]^GATC[CT]
1341 GGATCT
MseI 698 TTAA T^TAA
779 TTAA
Tru9I 698 TTAA T^TAA
779 TTAA
XhoII 83 AGATCT [GA]^GATC[CT]
1341 GGATCT
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I TspRI TthHB8I