Restriction map for
pFN21AE5668
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BsrI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 584 ACACGT A^C[GA][CT]GT
AhdI 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1037 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 538 AGCGCT AGC^GCT
ApaI 137 GGGCCC GGGCC^C
AspEI 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1068 GGACC G^G[AT]CC
BalI 1169 TGGCCA TGG^CCA
BanI 862 GGCACC G^G[CT][GA]CC
BglI 1496 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1252 GAGACC GGTCTC(1/5)
BsmFI 637 GGGAC GGGAC(10/14)
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BsrDI 644 GCAATG GCAATG(2/0)
BsrFI 42 GCCGGT [GA]^CCGG[CT]
BstAPI 1036 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 721 TTCGAA TT^CGAA
BstXI 1160 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 42 GCCGGT [GA]^CCGG[CT]
DraII 137 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 270 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 610 CGGCCG C^GGCCG
Eam1105I 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 610 CGGCCG C^GGCCG
Eco47III 538 AGCGCT AGC^GCT
Eco52I 610 CGGCCG C^GGCCG
EcoO109I 137 GGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 538 AGCGCT [GA]GCGC^[CT]
KspI 613 CCGCGG CCGC^GG
MluNI 1169 TGGCCA TGG^CCA
MscI 1169 TGGCCA TGG^CCA
MseI 1382 TTAA T^TAA
NotI 609 GCGGCCGC GC^GGCCGC
NspV 721 TTCGAA TT^CGAA
PflFI 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1354 GAGCTC GAGCT^C
SacII 613 CCGCGG CCGC^GG
SexAI 953 ACCTGGT A^CC[AT]GGT
SfuI 721 TTCGAA TT^CGAA
Tru9I 1382 TTAA T^TAA
Tth111I 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 777 CCAGCTGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 714 GACGTC GACGT^C
1468 GACGTC
AccII 614 CGCG CG^CG
947 CGCG
Alw44I 180 GTGCAC G^TGCAC
982 GTGCAC
ApaLI 180 GTGCAC G^TGCAC
982 GTGCAC
AvaI 594 CTCGGG C^[CT]CG[GA]G
1556 CCCGAG
BbvCI 1154 CCTCAGC CCTCAGC(-5/-2)
1455 CCTCAGC
BcnI 522 CCGGG CC^[GC]GG
1514 CCGGG
BsiEI 474 CGACCG CG[GA][CT]^CG
610 CGGCCG
BsmBI 712 GAGACG CGTCTC(1/5)
943 GAGACG
BsoBI 594 CTCGGG C^[CT]CG[GA]G
1556 CCCGAG
BstUI 614 CGCG CG^CG
947 CGCG
CfoI 539 GCGC GCG^C
948 GCGC
EaeI 610 CGGCCG [CT]^GGCC[GA]
1169 TGGCCA
EcoT14I 881 CCATGG C^C[AT][AT]GG
1130 CCATGG
FauI 1109 CCCGC CCCGC(4/6)
126 GCGGG
HgaI 945 GACGC GACGC(5/10)
35 GCGTC
HhaI 539 GCGC GCG^C
948 GCGC
HinP1I 539 GCGC G^CGC
948 GCGC
HinfI 886 GACTC G^A[ACGT]TC
907 GACTC
MvnI 614 CGCG CG^CG
947 CGCG
NciI 522 CCGGG CC^[GC]GG
1514 CCGGG
NcoI 881 CCATGG C^CATGG
1130 CCATGG
PleI 886 GACTC GAGTC(4/5)
907 GACTC
PstI 412 CTGCAG CTGCA^G
534 CTGCAG
PvuII 400 CAGCTG CAG^CTG
778 CAGCTG
ScaI 197 AGTACT AGT^ACT
1076 AGTACT
StuI 56 AGGCCT AGG^CCT
1271 AGGCCT
StyI 881 CCATGG C^C[AT][AT]GG
1130 CCATGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI BanII BbsI BbvI BcgI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII Hin1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I XhoII