Restriction map for
pFN21AE1868
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tru9I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 925 GTCTAC GT^[AC][GT]AC
AlwI 462 GGATC GGATC(4/5)
AspHI 1063 GTGCTC G[AT]GC[AT]^C
AvaII 237 GGTCC G^G[AT]CC
AviII 188 TGCGCA TGC^GCA
BanI 704 GGCGCC G^G[CT][GA]CC
BbeI 704 GGCGCC GGCGC^C
BbvCI 468 GCTGAGG CCTCAGC(-5/-2)
BfaI 175 CTAG C^TAG
BseRI 7 GAGGAG GAGGAG(10/8)
BsiHKAI 1063 GTGCTC G[AT]GC[AT]^C
BsmI 677 GAATGC GAATGC(1/-1)
BsrBI 829 GAGCGG CCGCTC(-3/-3)
DpnII 463 GATC ^GATC
FauI 43 GCGGG CCCGC(4/6)
FspI 188 TGCGCA TGC^GCA
KasI 704 GGCGCC G^GCGCC
KspI 19 CCGCGG CCGC^GG
MaeI 175 CTAG C^TAG
MboI 463 GATC ^GATC
MboII 848 TCTTC GAAGA(8/7)
MslI 578 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 704 GGCGCC GG^CGCC
NdeII 463 GATC ^GATC
NotI 299 GCGGCCGC GC^GGCCGC
NspI 778 ACATGC [GA]CATG^[CT]
PflMI 600 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 19 CCGCGG CCGC^GG
Sau3AI 463 GATC ^GATC
SfoI 704 GGCGCC GGC^GCC
SmaI 659 CCCGGG CCC^GGG
StuI 177 AGGCCT AGG^CCT
Tsp45I 202 GTGAC ^GT[GC]AC
Tsp509I 871 AATT ^AATT
Van91I 600 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1010 CCAGCCGCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 659 CCCGGG C^CCGGG
XmaI 659 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 207 GGCGTC G[GA]^CG[CT]C
704 GGCGCC
AlwNI 220 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
493 CAGGCACTG
Aor51HI 269 AGCGCT AGC^GCT
1085 AGCGCT
AspI 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
204 GACGGCGTC
BsaHI 207 GGCGTC G[GA]^CG[CT]C
704 GGCGCC
BsaI 959 GAGACC GGTCTC(1/5)
46 GGTCTC
BsgI 226 CTGCAC GTGCAG(16/14)
66 GTGCAG
BsiEI 16 CGGCCG CG[GA][CT]^CG
300 CGGCCG
BsrDI 1093 CATTGC GCAATG(2/0)
83 GCAATG
BsrI 1106 ACTGG ACTGG(1/-1)
768 CCAGT
BssSI 241 CACGAG CACGAG(-5/-1)
1076 CACGAG
DraIII 135 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1057 CACAACGTG
EagI 16 CGGCCG C^GGCCG
300 CGGCCG
EclXI 16 CGGCCG C^GGCCG
300 CGGCCG
Eco47III 269 AGCGCT AGC^GCT
1085 AGCGCT
Eco52I 16 CGGCCG C^GGCCG
300 CGGCCG
Hin1I 207 GGCGTC G[GA]^CG[CT]C
704 GGCGCC
MaeII 200 ACGT A^CGT
1061 ACGT
PflFI 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
204 GACGGCGTC
Tth111I 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
204 GACGGCGTC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EaeI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PstI PvuII Sau96I ScrFI SfcI
SmlI TfiI TseI TspRI