Restriction map for
pFN21AE2261
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspEI BsrFI BsrGI
BssHII BstEII BstPI CelII Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI HaeII HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 190 GTATAC GT^[AC][GT]AC
AcyI 676 GACGCC G[GA]^CG[CT]C
AflIII 306 ACATGT A^C[GA][CT]GT
Alw44I 477 GTGCAC G^TGCAC
AocI 340 CCTCAGG CC^T[ACGT]AGG
ApaI 1924 GGGCCC GGGCC^C
ApaLI 477 GTGCAC G^TGCAC
AviII 1445 TGCGCA TGC^GCA
BbsI 1419 GTCTTC GAAGAC(2/6)
BpuAI 1419 GTCTTC GAAGAC(2/6)
BsaHI 676 GACGCC G[GA]^CG[CT]C
BsgI 923 GTGCAG GTGCAG(16/14)
BsmBI 159 CGTCTC CGTCTC(1/5)
BsmI 599 GCATTC GAATGC(1/-1)
BspDI 200 ATCGAT AT^CGAT
BspHI 1284 TCATGA T^CATGA
BspLU11I 306 ACATGT A^CATGT
BsrBI 1342 GAGCGG CCGCTC(-3/-3)
BsrDI 885 CATTGC GCAATG(2/0)
BssSI 1895 CTCGTG CACGAG(-5/-1)
Bst1107I 190 GTATAC GTA^TAC
BstAPI 1255 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1908 TTCGAA TT^CGAA
BstXI 293 CCAAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 190 GTATAC GTA^TAC
Bsu36I 340 CCTCAGG CC^T[ACGT]AGG
ClaI 200 ATCGAT AT^CGAT
Eco81I 340 CCTCAGG CC^T[ACGT]AGG
EcoT14I 689 CCTTGG C^C[AT][AT]GG
FspI 1445 TGCGCA TGC^GCA
Hin1I 676 GACGCC G[GA]^CG[CT]C
MslI 460 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1256 CATATG CA^TATG
NspV 1908 TTCGAA TT^CGAA
PflMI 1093 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1722 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1284 TCATGA T^CATGA
SacI 867 GAGCTC GAGCT^C
SapI 869 GCTCTTC GCTCTTC(1/4)
ScaI 1780 AGTACT AGT^ACT
SfuI 1908 TTCGAA TT^CGAA
SpeI 1051 ACTAGT A^CTAGT
StyI 689 CCTTGG C^C[AT][AT]GG
Van91I 1093 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 1177 CGCG CG^CG
1179 CGCG
AlwI 633 GATCC GGATC(4/5)
1630 GATCC
Asp700I 346 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2044 GAATTTGTTC
AvaI 44 CTCGAG C^[CT]CG[GA]G
2097 CTCGAG
BanII 867 GAGCTC G[GA]GC[CT]^C
1924 GGGCCC
BglII 393 AGATCT A^GATCT
2101 AGATCT
BsmFI 1722 GGGAC GGGAC(10/14)
1900 GTCCC
BsoBI 44 CTCGAG C^[CT]CG[GA]G
2097 CTCGAG
BspMI 889 GCAGGT ACCTGC(4/8)
1399 GCAGGT
BstUI 1177 CGCG CG^CG
1179 CGCG
CfoI 1178 GCGC GCG^C
1446 GCGC
DraII 1722 GGGACCC [GA]G^G[ACGT]CC[CT]
1923 AGGGCCC
EaeI 122 CGGCCA [CT]^GGCC[GA]
2125 CGGCCA
EcoO109I 1722 GGGACCC [GA]G^G[ACGT]CC[CT]
1923 AGGGCCC
HhaI 1178 GCGC GCG^C
1446 GCGC
HinP1I 1178 GCGC G^CGC
1446 GCGC
HincII 673 GTTGAC GT[CT]^[GA]AC
906 GTCAAC
HindII 673 GTTGAC GT[CT]^[GA]AC
906 GTCAAC
MaeII 158 ACGT A^CGT
1620 ACGT
MvnI 1177 CGCG CG^CG
1179 CGCG
PaeR7I 44 CTCGAG C^TCGAG
2097 CTCGAG
PleI 1533 GACTC GAGTC(4/5)
952 GAGTC
PstI 565 CTGCAG CTGCA^G
1463 CTGCAG
SfaNI 1836 GATGC GCATC(5/9)
198 GCATC
SmlI 44 CTCGAG C^T[CT][GA]AG
2097 CTCGAG
SphI 778 GCATGC GCATG^C
1185 GCATGC
XcmI 680 CCACTGTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1593 CCAACTTCCAGTTGG
XhoI 44 CTCGAG C^TCGAG
2097 CTCGAG
XmnI 346 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2044 GAATTTGTTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbvI BclI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII