Restriction map for
pFN21AE3974
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MboI MboII MflI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AlwNI 191 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 59 AGCGCT AGC^GCT
BalI 689 TGGCCA TGG^CCA
BanI 670 GGCACC G^G[CT][GA]CC
BcgI 231 CGAGGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 695 GAGACC GGTCTC(1/5)
BseRI 628 CTCCTC GAGGAG(10/8)
BsgI 144 CTGCAC GTGCAG(16/14)
BsmAI 695 GAGAC GTCTC(1/5)
BssSI 680 CACGAG CACGAG(-5/-1)
EaeI 689 TGGCCA [CT]^GGCC[GA]
Eco47III 59 AGCGCT AGC^GCT
HaeII 59 AGCGCT [GA]GCGC^[CT]
HphI 234 GGTGA GGTGA(8/7)
MfeI 74 CAATTG C^AATTG
MluNI 689 TGGCCA TGG^CCA
MscI 689 TGGCCA TGG^CCA
MslI 100 CATGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 465 CAGCTG C[AC]G^C[GT]G
MunI 74 CAATTG C^AATTG
PflMI 8 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 465 CAGCTG CAG^CTG
SacI 625 GAGCTC GAGCT^C
SmlI 490 CTTGAG C^T[CT][GA]AG
Van91I 8 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 625 GAGCTC G[AT]GC[AT]^C
729 GTGCTC
AvaII 114 GGACC G^G[AT]CC
306 GGTCC
BanII 404 GGGCTC G[GA]GC[CT]^C
625 GAGCTC
BfaI 412 CTAG C^TAG
583 CTAG
BlpI 462 GCTCAGC GC^T[ACGT]AGC
731 GCTCAGC
BpmI 737 CTCCAG CTGGAG(16/14)
790 CTCCAG
Bpu1102I 462 GCTCAGC GC^T[ACGT]AGC
731 GCTCAGC
BsiHKAI 625 GAGCTC G[AT]GC[AT]^C
729 GTGCTC
BsmFI 591 GGGAC GGGAC(10/14)
786 GGGAC
BsmI 237 GAATGC GAATGC(1/-1)
32 GCATTC
BsrBI 460 CCGCTC CCGCTC(-3/-3)
417 GAGCGG
BstXI 332 CCAATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
361 CCACTTCACTGG
CelII 462 GCTCAGC GC^T[ACGT]AGC
731 GCTCAGC
MaeI 412 CTAG C^TAG
583 CTAG
MaeIII 210 GTTAC ^GT[ACGT]AC
852 GTTAC
MseI 64 TTAA T^TAA
380 TTAA
PpuMI 113 AGGACCT [GA]G^G[AT]CC[CT]
305 AGGTCCT
Tru9I 64 TTAA T^TAA
380 TTAA
Tsp509I 75 AATT ^AATT
656 AATT
XcmI 560 CCATCTCCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
633 CCACAGAGCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MnlI MvaI MwoI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TseI TspRI