Restriction map for
pFN21AE2204
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII AccI AccIII AclI AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII BciVI
BglII BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HgaI HindIII HpaI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1861 GGTACC G^GTACC
AcyI 2738 GGCGCC G[GA]^CG[CT]C
AflII 153 CTTAAG C^TTAAG
Alw44I 1810 GTGCAC G^TGCAC
AocI 477 CCTGAGG CC^T[ACGT]AGG
ApaLI 1810 GTGCAC G^TGCAC
Asp700I 2199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1861 GGTACC G^GTACC
BbeI 2738 GGCGCC GGCGC^C
BbrPI 1951 CACGTG CAC^GTG
BcgI 294 CGAAGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 153 CTTAAG C^TTAAG
BsaBI 1689 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2738 GGCGCC G[GA]^CG[CT]C
BsaI 771 GGTCTC GGTCTC(1/5)
BsiEI 846 CGACCG CG[GA][CT]^CG
BsrBI 553 GAGCGG CCGCTC(-3/-3)
BstXI 2268 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 477 CCTGAGG CC^T[ACGT]AGG
DrdI 1735 GACGGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1139 CCGCGG C^C[GA][CT]GG
Eco81I 477 CCTGAGG CC^T[ACGT]AGG
EcoRI 2017 GAATTC G^AATTC
Hin1I 2738 GGCGCC G[GA]^CG[CT]C
HincII 2516 GTCAAC GT[CT]^[GA]AC
HindII 2516 GTCAAC GT[CT]^[GA]AC
KasI 2738 GGCGCC G^GCGCC
KpnI 1861 GGTACC GGTAC^C
KspI 1139 CCGCGG CCGC^GG
MamI 1689 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1206 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2617 GCCGGC GCC^GGC
NarI 2738 GGCGCC GG^CGCC
NgoMIV 2617 GCCGGC G^CCGGC
PmaCI 1951 CACGTG CAC^GTG
PmlI 1951 CACGTG CAC^GTG
PshAI 1648 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1139 CCGCGG CCGC^GG
SfiI 2170 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2738 GGCGCC GGC^GCC
XcmI 2003 CCAACCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 1140 CGCG CG^CG
1927 CGCG
AcsI 1348 AAATTC [GA]^AATT[CT]
2017 GAATTC
ApaI 170 GGGCCC GGGCC^C
2303 GGGCCC
ApoI 1348 AAATTC [GA]^AATT[CT]
2017 GAATTC
AvrII 822 CCTAGG C^CTAGG
2364 CCTAGG
BalI 2277 TGGCCA TGG^CCA
2607 TGGCCA
BamHI 2625 GGATCC G^GATCC
2802 GGATCC
BclI 1169 TGATCA T^GATCA
1508 TGATCA
BlnI 822 CCTAGG C^CTAGG
2364 CCTAGG
BlpI 550 GCTGAGC GC^T[ACGT]AGC
1921 GCTGAGC
Bpu1102I 550 GCTGAGC GC^T[ACGT]AGC
1921 GCTGAGC
BsaAI 1273 TACGTG [CT]AC^GT[GA]
1951 CACGTG
BsmI 1834 GAATGC GAATGC(1/-1)
2069 GCATTC
BspMI 2135 ACCTGC ACCTGC(4/8)
2238 GCAGGT
BsrDI 1851 CATTGC GCAATG(2/0)
2668 CATTGC
BssSI 877 CACGAG CACGAG(-5/-1)
958 CACGAG
BstUI 1140 CGCG CG^CG
1927 CGCG
CelII 550 GCTGAGC GC^T[ACGT]AGC
1921 GCTGAGC
FbaI 1169 TGATCA T^GATCA
1508 TGATCA
HaeII 473 AGCGCC [GA]GCGC^[CT]
2738 GGCGCC
MluNI 2277 TGGCCA TGG^CCA
2607 TGGCCA
MscI 2277 TGGCCA TGG^CCA
2607 TGGCCA
MseI 154 TTAA T^TAA
1376 TTAA
MvnI 1140 CGCG CG^CG
1927 CGCG
PpuMI 371 GGGACCT [GA]G^G[AT]CC[CT]
2858 AGGACCC
SapI 741 GAAGAGC GCTCTTC(1/4)
1500 GAAGAGC
SmaI 1040 CCCGGG CCC^GGG
1562 CCCGGG
SmlI 153 CTTAAG C^T[CT][GA]AG
457 CTCAAG
StuI 316 AGGCCT AGG^CCT
2270 AGGCCT
Tru9I 154 TTAA T^TAA
1376 TTAA
XmaCI 1040 CCCGGG C^CCGGG
1562 CCCGGG
XmaI 1040 CCCGGG C^CCGGG
1562 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SphI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII