Restriction map for
pFN21AE9440
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 806 GTAGAC GT^[AC][GT]AC
AcyI 49 GACGCC G[GA]^CG[CT]C
AspI 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 896 CCCGAG C^[CT]CG[GA]G
BalI 86 TGGCCA TGG^CCA
BbvCI 913 GCTGAGG CCTCAGC(-5/-2)
BcnI 717 CCCGG CC^[GC]GG
BfaI 340 CTAG C^TAG
BsaHI 49 GACGCC G[GA]^CG[CT]C
BsiEI 719 CGGTCG CG[GA][CT]^CG
BsiWI 270 CGTACG C^GTACG
BsmBI 558 CGTCTC CGTCTC(1/5)
BsmI 788 GCATTC GAATGC(1/-1)
BsoBI 896 CCCGAG C^[CT]CG[GA]G
BspHI 452 TCATGA T^CATGA
BstAPI 830 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 738 GGTCACC G^GT[ACGT]ACC
BstPI 738 GGTCACC G^GT[ACGT]ACC
DsaI 61 CCATGG C^C[GA][CT]GG
EaeI 86 TGGCCA [CT]^GGCC[GA]
EcoNI 820 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 738 GGTCACC G^GT[ACGT]ACC
EcoT14I 61 CCATGG C^C[AT][AT]GG
FauI 684 CCCGC CCCGC(4/6)
HapII 718 CCGG C^CGG
HgaI 49 GACGC GACGC(5/10)
Hin1I 49 GACGCC G[GA]^CG[CT]C
HpaII 718 CCGG C^CGG
HphI 740 TCACC GGTGA(8/7)
MaeI 340 CTAG C^TAG
MluNI 86 TGGCCA TGG^CCA
MscI 86 TGGCCA TGG^CCA
MseI 931 TTAA T^TAA
MspI 718 CCGG C^CGG
NciI 717 CCCGG CC^[GC]GG
NcoI 61 CCATGG C^CATGG
NdeI 938 CATATG CA^TATG
NlaIV 57 GGGGCC GG[ACGT]^[ACGT]CC
PflFI 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 743 CCAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 159 CTGCAG CTGCA^G
RcaI 452 TCATGA T^CATGA
StyI 61 CCATGG C^C[AT][AT]GG
Tru9I 931 TTAA T^TAA
Tth111I 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 743 CCAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 685 CCGC CCGC(-3/-1)
958 CCGC
Bsp1407I 2 TGTACA T^GTACA
767 TGTACA
BsrGI 2 TGTACA T^GTACA
767 TGTACA
BstF5I 990 CATCC GGATG(2/0)
921 GGATG
BstYI 149 AGATCC [GA]^GATC[CT]
659 GGATCT
Cac8I 491 GCCAGC GC[ACGT]^[ACGT]GC
835 GCCTGC
DrdI 712 GACGACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
715 GACCCGGTCGTC
FokI 990 CATCC GGATG(9/13)
921 GGATG
MflI 149 AGATCC [GA]^GATC[CT]
659 GGATCT
MslI 645 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
977 CACAAGAGTG
SapI 216 GCTCTTC GCTCTTC(1/4)
630 GCTCTTC
SfcI 159 CTGCAG C^T[GA][CT]AG
795 CTACAG
SspBI 2 TGTACA T^GTACA
767 TGTACA
TaqI 17 TCGA T^CGA
245 TCGA
Tsp509I 111 AATT ^AATT
384 AATT
TthHB8I 17 TCGA T^CGA
245 TCGA
XhoII 149 AGATCC [GA]^GATC[CT]
659 GGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI AspHI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstNI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI