Restriction map for
pFN21AB8826
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 291 GTCTAC GT^[AC][GT]AC
AgeI 485 ACCGGT A^CCGGT
AlwI 563 GGATC GGATC(4/5)
AspI 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 184 CCCGGG C^[CT]CG[GA]G
BcgI 353 GCACGCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 455 AGATCT A^GATCT
BlpI 883 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 883 GCTCAGC GC^T[ACGT]AGC
BsaI 312 GGTCTC GGTCTC(1/5)
BsaWI 485 ACCGGT [AT]^CCGG[AT]
BsgI 81 CTGCAC GTGCAG(16/14)
BsoBI 184 CCCGGG C^[CT]CG[GA]G
BsrDI 1064 GCAATG GCAATG(2/0)
BsrFI 485 ACCGGT [GA]^CCGG[CT]
CelII 883 GCTCAGC GC^T[ACGT]AGC
Cfr10I 485 ACCGGT [GA]^CCGG[CT]
Cfr13I 1211 GGCCC G^G[ACGT]CC
DraIII 169 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 951 GATATC GAT^ATC
HgaI 393 GACGC GACGC(5/10)
HindIII 1201 AAGCTT A^AGCTT
MslI 895 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 78 CAGCTG C[AC]G^C[GT]G
PflFI 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 678 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 485 ACCGGT A^CCGGT
PvuII 78 CAGCTG CAG^CTG
Sau96I 1211 GGCCC G^G[ACGT]CC
SmaI 184 CCCGGG CCC^GGG
TaqI 1162 TCGA T^CGA
Tsp45I 583 GTGAC ^GT[GC]AC
Tth111I 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1162 TCGA T^CGA
Van91I 678 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 184 CCCGGG C^CCGGG
XmaI 184 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 75 CCGC CCGC(-3/-1)
553 GCGG
AspHI 136 GAGCAC G[AT]GC[AT]^C
592 GTGCTC
BanI 60 GGTGCC G^G[CT][GA]CC
893 GGCACC
BcnI 184 CCCGG CC^[GC]GG
185 CCGGG
BpmI 370 CTCCAG CTGGAG(16/14)
443 CTCCAG
BsiHKAI 136 GAGCAC G[AT]GC[AT]^C
592 GTGCTC
BsmAI 1027 GAGAC GTCTC(1/5)
313 GTCTC
BsmFI 617 GGGAC GGGAC(10/14)
799 GTCCC
BstYI 455 AGATCT [GA]^GATC[CT]
563 GGATCT
DsaI 317 CCATGG C^C[GA][CT]GG
1136 CCATGG
HapII 185 CCGG C^CGG
486 CCGG
HpaII 185 CCGG C^CGG
486 CCGG
HphI 777 GGTGA GGTGA(8/7)
377 TCACC
MflI 455 AGATCT [GA]^GATC[CT]
563 GGATCT
MspI 185 CCGG C^CGG
486 CCGG
NciI 184 CCCGG CC^[GC]GG
185 CCGGG
NcoI 317 CCATGG C^CATGG
1136 CCATGG
NspI 322 GCATGT [GA]CATG^[CT]
1037 GCATGT
TfiI 219 GATTC G^A[AT]TC
699 GATTC
XcmI 299 CCAACTACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
372 CCAGTTCACCACTGG
XhoII 455 AGATCT [GA]^GATC[CT]
563 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI BalI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI ScrFI
SfaNI StyI Tru9I TseI Tsp509I
TspRI