Restriction map for
pFN21AE5373
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BclI BcnI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 627 GCGG CCGC(-3/-1)
AcsI 137 AAATTC [GA]^AATT[CT]
AfaI 778 GTAC GT^AC
ApaI 403 GGGCCC GGGCC^C
ApoI 137 AAATTC [GA]^AATT[CT]
Asp700I 273 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 666 GGTCC G^G[AT]CC
BanII 403 GGGCCC G[GA]GC[CT]^C
BbvI 295 GCTGC GCAGC(8/12)
BciVI 810 GTATCC GTATCC(6/5)
BglII 119 AGATCT A^GATCT
BlpI 130 GCTGAGC GC^T[ACGT]AGC
BmyI 403 GGGCCC G[AGT]GC[ACT]^C
BpmI 159 CTCCAG CTGGAG(16/14)
Bpu1102I 130 GCTGAGC GC^T[ACGT]AGC
BsaI 156 GGTCTC GGTCTC(1/5)
BseRI 123 CTCCTC GAGGAG(10/8)
BsiYI 834 CCTGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 834 CCTGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 157 GTCTC GTCTC(1/5)
BsmI 507 GAATGC GAATGC(1/-1)
Bsp1286I 403 GGGCCC G[AGT]GC[ACT]^C
BsrI 101 ACTGG ACTGG(1/-1)
BssKI 260 CCTGG ^CC[ACGT]GG
BstNI 260 CCTGG CC^[AT]GG
CelII 130 GCTGAGC GC^T[ACGT]AGC
DsaI 443 CCATGG C^C[GA][CT]GG
EarI 366 GAAGAG CTCTTC(1/4)
EcoRII 260 CCTGG ^CC[AT]GG
HaeIII 404 GGCC GG^CC
HincII 106 GTTAAC GT[CT]^[GA]AC
HindII 106 GTTAAC GT[CT]^[GA]AC
HinfI 750 GAATC G^A[ACGT]TC
HpaI 106 GTTAAC GTT^AAC
HphI 390 GGTGA GGTGA(8/7)
Ksp632I 366 GAAGAG CTCTTC(1/4)
MspA1I 128 CAGCTG C[AC]G^C[GT]G
MvaI 260 CCTGG CC^[AT]GG
MwoI 250 GCTTCTAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 443 CCATGG C^CATGG
NheI 298 GCTAGC G^CTAGC
NspI 284 GCATGT [GA]CATG^[CT]
PvuII 128 CAGCTG CAG^CTG
RsaI 778 GTAC GT^AC
ScrFI 260 CCTGG CC^[ACGT]GG
SfaNI 624 GATGC GCATC(5/9)
TfiI 750 GAATC G^A[AT]TC
TseI 295 GCTGC G^C[AT]GC
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 273 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 652 GATCC GGATC(4/5)
643 GGATC
BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
303 CCTCAGC
BstF5I 831 CATCC GGATG(2/0)
447 GGATG
Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC
298 GCTAGC
DraII 402 GGGGCCC [GA]G^G[ACGT]CC[CT]
403 GGGCCCT
EcoO109I 402 GGGGCCC [GA]G^G[ACGT]CC[CT]
403 GGGCCCT
Fnu4HI 295 GCTGC GC^[ACGT]GC
627 GCGGC
FokI 831 CATCC GGATG(9/13)
447 GGATG
HindIII 525 AAGCTT A^AGCTT
677 AAGCTT
ItaI 295 GCTGC GC^[ACGT]GC
627 GCGGC
Tsp45I 484 GTGAC ^GT[GC]AC
691 GTGAC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BfaI BsaJI BstYI Cfr13I
DdeI DpnII EcoT14I MaeI MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIII NlaIV Sau3AI Sau96I
SmlI StyI Tru9I Tsp509I XhoII