Restriction map for
pFN21AE5357
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FokI FseI FspI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflIII 443 ACACGT A^C[GA][CT]GT
AlwNI 282 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 132 TGATCA T^GATCA
BglII 119 AGATCT A^GATCT
BsaAI 444 CACGTA [CT]AC^GT[GA]
BseRI 123 CTCCTC GAGGAG(10/8)
BsmI 507 GAATGC GAATGC(1/-1)
BspMI 885 GCAGGT ACCTGC(4/8)
BsrI 915 CCAGT ACTGG(1/-1)
BstXI 792 CCAAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 231 TTTAAA TTT^AAA
DraIII 427 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 633 CCATGG C^C[GA][CT]GG
EcoRV 576 GATATC GAT^ATC
EcoT14I 633 CCATGG C^C[AT][AT]GG
FbaI 132 TGATCA T^GATCA
HgaI 852 GACGC GACGC(5/10)
HincII 918 GTCAAC GT[CT]^[GA]AC
HindII 918 GTCAAC GT[CT]^[GA]AC
HindIII 794 AAGCTT A^AGCTT
MspA1I 128 CAGCTG C[AC]G^C[GT]G
NcoI 633 CCATGG C^CATGG
NheI 543 GCTAGC G^CTAGC
PvuII 128 CAGCTG CAG^CTG
SfcI 533 CTGTAG C^T[GA][CT]AG
SspI 183 AATATT AAT^ATT
StyI 633 CCATGG C^C[AT][AT]GG
TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 29 CCATTCTAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
303 CCTCAGC
BbvI 627 GCAGC GCAGC(8/12)
145 GCTGC
BpmI 159 CTCCAG CTGGAG(16/14)
339 CTCCAG
BsmAI 111 GAGAC GTCTC(1/5)
157 GTCTC
BsrDI 96 CATTGC GCAATG(2/0)
511 GCAATG
BssKI 101 CCTGG ^CC[ACGT]GG
260 CCTGG
BstNI 101 CCTGG CC^[AT]GG
260 CCTGG
Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC
543 GCTAGC
EcoRII 101 CCTGG ^CC[AT]GG
260 CCTGG
Fnu4HI 145 GCTGC GC^[ACGT]GC
627 GCAGC
HinfI 213 GAATC G^A[ACGT]TC
803 GAATC
ItaI 145 GCTGC GC^[ACGT]GC
627 GCAGC
MaeII 445 ACGT A^CGT
482 ACGT
MvaI 101 CCTGG CC^[AT]GG
260 CCTGG
MwoI 145 GCTGCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
538 GCCATGCTAGC
NlaIII 540 CATG CATG^
634 CATG
ScrFI 101 CCTGG CC^[ACGT]GG
260 CCTGG
SmlI 322 CTCAAG C^T[CT][GA]AG
647 CTCAAG
TfiI 213 GAATC G^A[AT]TC
803 GAATC
TseI 145 GCTGC G^C[AT]GC
627 GCAGC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BsaJI BstYI DdeI DpnII HphI
MaeI MaeIII MboI MboII MflI
MnlI MseI NdeII Sau3AI SfaNI
Tru9I Tsp45I Tsp509I XhoII