Restriction map for
pFN21AE5374
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MunI MvnI MwoI
NaeI NarI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 514 GTAC GT^AC
AlwI 643 GGATC GGATC(4/5)
Asp700I 242 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 666 GGTCC G^G[AT]CC
AvrII 754 CCTAGG C^CTAGG
BcnI 774 CCCGG CC^[GC]GG
BfaI 755 CTAG C^TAG
BlnI 754 CCTAGG C^CTAGG
BpmI 159 CTCCAG CTGGAG(16/14)
BsaBI 588 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 156 GGTCTC GGTCTC(1/5)
BsmAI 157 GTCTC GTCTC(1/5)
BsmI 817 GCATTC GAATGC(1/-1)
BstNI 260 CCTGG CC^[AT]GG
Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 666 GGTCC G^G[ACGT]CC
DraI 360 TTTAAA TTT^AAA
DrdI 772 GACCCGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 633 CCATGG C^C[GA][CT]GG
EarI 108 GAAGAG CTCTTC(1/4)
EcoRII 260 CCTGG ^CC[AT]GG
EcoRV 576 GATATC GAT^ATC
HapII 775 CCGG C^CGG
HindIII 677 AAGCTT A^AGCTT
HpaII 775 CCGG C^CGG
Ksp632I 108 GAAGAG CTCTTC(1/4)
MaeI 755 CTAG C^TAG
MamI 588 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 384 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 128 CAGCTG C[AC]G^C[GT]G
MspI 775 CCGG C^CGG
MvaI 260 CCTGG CC^[AT]GG
NciI 774 CCCGG CC^[GC]GG
NcoI 633 CCATGG C^CATGG
NspI 284 GCATGT [GA]CATG^[CT]
PvuII 128 CAGCTG CAG^CTG
RsaI 514 GTAC GT^AC
Sau96I 666 GGTCC G^G[ACGT]CC
TaqI 821 TCGA T^CGA
TfiI 213 GAATC G^A[AT]TC
TthHB8I 821 TCGA T^CGA
XmnI 242 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 154 GCGG CCGC(-3/-1)
765 GCGG
AcsI 90 AAATTT [GA]^AATT[CT]
137 AAATTC
ApoI 90 AAATTT [GA]^AATT[CT]
137 AAATTC
BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
303 CCTCAGC
BglII 119 AGATCT A^GATCT
651 AGATCT
BseRI 123 CTCCTC GAGGAG(10/8)
366 GAGGAG
BsmFI 403 GGGAC GGGAC(10/14)
570 GTCCC
BssKI 260 CCTGG ^CC[ACGT]GG
774 CCCGG
BstF5I 831 CATCC GGATG(2/0)
447 GGATG
BstYI 119 AGATCT [GA]^GATC[CT]
651 AGATCT
FokI 831 CATCC GGATG(9/13)
447 GGATG
HphI 105 GGTGA GGTGA(8/7)
390 GGTGA
MaeIII 268 GTAAC ^GT[ACGT]AC
535 GTAAC
MflI 119 AGATCT [GA]^GATC[CT]
651 AGATCT
PleI 823 GACTC GAGTC(4/5)
750 GAGTC
ScrFI 260 CCTGG CC^[ACGT]GG
774 CCCGG
SfaNI 588 GATGC GCATC(5/9)
624 GATGC
SmlI 322 CTCAAG C^T[CT][GA]AG
616 CTCAAG
TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^
144 CACTG
XhoII 119 AGATCT [GA]^GATC[CT]
651 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbvI BsaJI BsrI DdeI
DpnII EcoT14I Fnu4HI HinfI ItaI
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI StyI Tru9I TseI
Tsp509I