Restriction map for
pFN21AE5458
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BanI BbeI BbrPI BcgI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaI BsgI
BsiEI BsiHKAI BsiWI BsoBI Bsp1407I
BspDI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MfeI MluI MluNI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StyI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 655 TCCGGA T^CCGGA
AcyI 661 GACGCC G[GA]^CG[CT]C
AflIII 899 ACATGT A^C[GA][CT]GT
AocI 920 CCTCAGG CC^T[ACGT]AGG
AspI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 755 GGACC G^G[AT]CC
BamHI 137 GGATCC G^GATCC
BanII 916 GAGCCC G[GA]GC[CT]^C
BbsI 415 GTCTTC GAAGAC(2/6)
BbvCI 891 GCTGAGG CCTCAGC(-5/-2)
BciVI 472 GGATAC GTATCC(6/5)
BglII 119 AGATCT A^GATCT
BmyI 916 GAGCCC G[AGT]GC[ACT]^C
BpuAI 415 GTCTTC GAAGAC(2/6)
BsaBI 441 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 661 GACGCC G[GA]^CG[CT]C
BsaWI 655 TCCGGA [AT]^CCGG[AT]
BseAI 655 TCCGGA T^CCGGA
BsmBI 659 GAGACG CGTCTC(1/5)
BsmFI 579 GTCCC GGGAC(10/14)
BsmI 248 GAATGC GAATGC(1/-1)
Bsp1286I 916 GAGCCC G[AGT]GC[ACT]^C
BspEI 655 TCCGGA T^CCGGA
BspLU11I 899 ACATGT A^CATGT
BsrDI 586 GCAATG GCAATG(2/0)
BstF5I 447 CATCC GGATG(2/0)
Bsu36I 920 CCTCAGG CC^T[ACGT]AGG
Cac8I 664 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 755 GGACC G^G[ACGT]CC
DraI 426 TTTAAA TTT^AAA
EarI 123 CTCTTC CTCTTC(1/4)
Eco81I 920 CCTCAGG CC^T[ACGT]AGG
EcoNI 758 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 447 CATCC GGATG(9/13)
HapII 656 CCGG C^CGG
HgaI 661 GACGC GACGC(5/10)
Hin1I 661 GACGCC G[GA]^CG[CT]C
HpaII 656 CCGG C^CGG
Ksp632I 123 CTCTTC CTCTTC(1/4)
MamI 441 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 655 TCCGGA T^CCGGA
MspI 656 CCGG C^CGG
NspI 899 ACATGT [GA]CATG^[CT]
PflFI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 795 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 408 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 755 GGACC G^G[ACGT]CC
SfaNI 633 GATGC GCATC(5/9)
SfcI 299 CTACAG C^T[GA][CT]AG
SmlI 325 CTCAAG C^T[CT][GA]AG
TaqI 162 TCGA T^CGA
Tth111I 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 162 TCGA T^CGA
Van91I 795 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 311 GTAC GT^AC
897 GTAC
AlwI 138 GATCC GGATC(4/5)
137 GGATC
BbvI 636 GCAGC GCAGC(8/12)
396 GCTGC
BclI 272 TGATCA T^GATCA
698 TGATCA
BseRI 598 CTCCTC GAGGAG(10/8)
683 GAGGAG
BsiYI 758 CCTCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
795 CCAGGTGATGG
BslI 758 CCTCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
795 CCAGGTGATGG
BsmAI 659 GAGAC GTCTC(1/5)
861 GAGAC
BstYI 119 AGATCT [GA]^GATC[CT]
137 GGATCC
FbaI 272 TGATCA T^GATCA
698 TGATCA
Fnu4HI 396 GCTGC GC^[ACGT]GC
636 GCAGC
HaeIII 304 GGCC GG^CC
867 GGCC
HphI 87 GGTGA GGTGA(8/7)
798 GGTGA
ItaI 396 GCTGC GC^[ACGT]GC
636 GCAGC
MflI 119 AGATCT [GA]^GATC[CT]
137 GGATCC
MwoI 220 GCTTTATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
882 GCTACTGTGGC
NlaIV 137 GGATCC GG[ACGT]^[ACGT]CC
915 GGAGCC
RsaI 311 GTAC GT^AC
897 GTAC
SspI 183 AATATT AAT^ATT
430 AATATT
StuI 303 AGGCCT AGG^CCT
866 AGGCCT
TseI 396 GCTGC G^C[AT]GC
636 GCAGC
XhoII 119 AGATCT [GA]^GATC[CT]
137 GGATCC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsrI
BssKI BstNI DdeI DpnII EcoRII
HinfI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
Sau3AI ScrFI TfiI Tru9I Tsp45I
Tsp509I TspRI