Restriction map for
pFN21AE5325
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 82 GTAC GT^AC
AspI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvI 886 GCAGC GCAGC(8/12)
BclI 86 TGATCA T^GATCA
BmyI 8 GTGCCC G[AGT]GC[ACT]^C
BsaI 659 GAGACC GGTCTC(1/5)
BseRI 139 CTCCTC GAGGAG(10/8)
BsiYI 161 CCAGAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 161 CCAGAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 799 GAGACG CGTCTC(1/5)
Bsp1286I 8 GTGCCC G[AGT]GC[ACT]^C
BsrDI 68 GCAATG GCAATG(2/0)
BssKI 665 CCAGG ^CC[ACGT]GG
BstNI 665 CCAGG CC^[AT]GG
Cac8I 294 GCTTGC GC[ACGT]^[ACGT]GC
DraI 426 TTTAAA TTT^AAA
EcoRI 46 GAATTC G^AATTC
EcoRII 665 CCAGG ^CC[AT]GG
EcoT22I 448 ATGCAT ATGCA^T
FbaI 86 TGATCA T^GATCA
Fnu4HI 886 GCAGC GC^[ACGT]GC
ItaI 886 GCAGC GC^[ACGT]GC
MaeIII 509 GTGAC ^GT[ACGT]AC
MvaI 665 CCAGG CC^[AT]GG
NlaIV 406 GGAACC GG[ACGT]^[ACGT]CC
NsiI 448 ATGCAT ATGCA^T
PflFI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 809 GAGTC GAGTC(4/5)
PshAI 669 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 613 CTGCAG CTGCA^G
RsaI 82 GTAC GT^AC
ScaI 81 AGTACT AGT^ACT
ScrFI 665 CCAGG CC^[ACGT]GG
SfaNI 311 GCATC GCATC(5/9)
SfcI 613 CTGCAG C^T[GA][CT]AG
SspI 183 AATATT AAT^ATT
StuI 869 AGGCCT AGG^CCT
TaqI 131 TCGA T^CGA
TseI 886 GCAGC G^C[AT]GC
Tsp45I 509 GTGAC ^GT[GC]AC
Tth111I 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 131 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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BbsI 415 GTCTTC GAAGAC(2/6)
811 GTCTTC
BpmI 159 CTCCAG CTGGAG(16/14)
625 CTCCAG
BpuAI 415 GTCTTC GAAGAC(2/6)
811 GTCTTC
BsmAI 659 GAGAC GTCTC(1/5)
799 GAGAC
BsrI 101 ACTGG ACTGG(1/-1)
461 ACTGG
EcoT14I 410 CCTTGG C^C[AT][AT]GG
671 CCAAGG
HaeIII 579 GGCC GG^CC
870 GGCC
MnlI 125 CCTC CCTC(7/6)
141 CCTC
MslI 222 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
679 CATACAAATG
NspI 446 ACATGC [GA]CATG^[CT]
788 ACATGC
StyI 410 CCTTGG C^C[AT][AT]GG
671 CCAAGG
TfiI 387 GATTC G^A[AT]TC
843 GATTC
TspRI 527 CAGTG CA[GC]TG[ACGT][ACGT]^
779 CAGTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AluI ApoI AvaII BfaI
BsaJI Cfr13I DdeI DpnII HinfI
MaeI MboI MboII MseI NdeII
NlaIII Sau3AI Sau96I Tru9I Tsp509I