Restriction map for
pFN21AE1220
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MamI MluI MluNI
MroI MscI MspI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 548 GCGG CCGC(-3/-1)
AcsI 386 AAATTT [GA]^AATT[CT]
AcyI 163 GGCGTC G[GA]^CG[CT]C
ApoI 386 AAATTT [GA]^AATT[CT]
BbrPI 675 CACGTG CAC^GTG
BbsI 72 GTCTTC GAAGAC(2/6)
BciVI 634 GGATAC GTATCC(6/5)
BpuAI 72 GTCTTC GAAGAC(2/6)
BsaHI 163 GGCGTC G[GA]^CG[CT]C
BseRI 573 CTCCTC GAGGAG(10/8)
BsiYI 580 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 580 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 177 GCAGGT ACCTGC(4/8)
BsrDI 189 CATTGC GCAATG(2/0)
BssKI 586 CCTGG ^CC[ACGT]GG
BssSI 4 CACGAG CACGAG(-5/-1)
BstNI 586 CCTGG CC^[AT]GG
DdeI 533 CTCAG C^T[ACGT]AG
DraI 423 TTTAAA TTT^AAA
EcoRII 586 CCTGG ^CC[AT]GG
HaeIII 589 GGCC GG^CC
Hin1I 163 GGCGTC G[GA]^CG[CT]C
HinfI 351 GATTC G^A[ACGT]TC
HphI 111 GGTGA GGTGA(8/7)
MaeIII 371 GTTAC ^GT[ACGT]AC
MfeI 345 CAATTG C^AATTG
MslI 189 CATTGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 546 CAGCGG C[AC]G^C[GT]G
MunI 345 CAATTG C^AATTG
MvaI 586 CCTGG CC^[AT]GG
NspI 94 GCATGC [GA]CATG^[CT]
PmaCI 675 CACGTG CAC^GTG
PmlI 675 CACGTG CAC^GTG
PstI 669 CTGCAG CTGCA^G
ScrFI 586 CCTGG CC^[ACGT]GG
SfcI 669 CTGCAG C^T[GA][CT]AG
SmlI 489 CTTGAG C^T[CT][GA]AG
SphI 94 GCATGC GCATG^C
SspI 281 AATATT AAT^ATT
TfiI 351 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 608 GATCC GGATC(4/5)
713 GATCC
BmyI 230 GGGCAC G[AGT]GC[ACT]^C
266 GTGCCC
BsaAI 637 TACGTG [CT]AC^GT[GA]
675 CACGTG
BsmAI 379 GTCTC GTCTC(1/5)
531 GTCTC
Bsp1286I 230 GGGCAC G[AGT]GC[ACT]^C
266 GTGCCC
BstYI 607 AGATCC [GA]^GATC[CT]
712 AGATCC
DpnII 608 GATC ^GATC
713 GATC
EarI 26 CTCTTC CTCTTC(1/4)
255 GAAGAG
HgaI 551 GACGC GACGC(5/10)
164 GCGTC
Ksp632I 26 CTCTTC CTCTTC(1/4)
255 GAAGAG
MaeII 638 ACGT A^CGT
676 ACGT
MboI 608 GATC ^GATC
713 GATC
MflI 607 AGATCC [GA]^GATC[CT]
712 AGATCC
NdeII 608 GATC ^GATC
713 GATC
NlaIV 433 GGTTCC GG[ACGT]^[ACGT]CC
571 GGCTCC
Sau3AI 608 GATC ^GATC
713 GATC
SfaNI 195 GATGC GCATC(5/9)
148 GCATC
XhoII 607 AGATCC [GA]^GATC[CT]
712 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BbvI BsrI BstF5I BstXI
Cac8I Fnu4HI FokI ItaI MboII
MnlI MseI NlaIII Tru9I TseI
Tsp509I TspRI