Restriction map for
pFN21AE5157
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglII BlnI BmyI BpmI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MboII MfeI
MluI MroI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 60 CGCG CG^CG
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflII 235 CTTAAG C^TTAAG
Aor51HI 34 AGCGCT AGC^GCT
AvaI 116 CTCGGG C^[CT]CG[GA]G
AvaII 54 GGTCC G^G[AT]CC
AviII 101 TGCGCA TGC^GCA
BalI 170 TGGCCA TGG^CCA
BamHI 273 GGATCC G^GATCC
BcnI 87 CCCGG CC^[GC]GG
BfrI 235 CTTAAG C^TTAAG
BglI 290 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 37 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 37 GCTGAGC GC^T[ACGT]AGC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsmI 93 GAATGC GAATGC(1/-1)
BsoBI 116 CTCGGG C^[CT]CG[GA]G
BsrFI 21 ACCGGC [GA]^CCGG[CT]
BstAPI 153 GCAATTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 60 CGCG CG^CG
CelII 37 GCTGAGC GC^T[ACGT]AGC
Cfr10I 21 ACCGGC [GA]^CCGG[CT]
Eco47III 34 AGCGCT AGC^GCT
EcoNI 57 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 342 CCATGG C^C[AT][AT]GG
FspI 101 TGCGCA TGC^GCA
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HphI 66 GGTGA GGTGA(8/7)
MluNI 170 TGGCCA TGG^CCA
MscI 170 TGGCCA TGG^CCA
MseI 236 TTAA T^TAA
MspA1I 213 CAGCTG C[AC]G^C[GT]G
MvnI 60 CGCG CG^CG
NciI 87 CCCGG CC^[GC]GG
NcoI 342 CCATGG C^CATGG
NlaIV 273 GGATCC GG[ACGT]^[ACGT]CC
PflMI 337 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 213 CAGCTG CAG^CTG
SfaNI 147 GATGC GCATC(5/9)
StyI 342 CCATGG C^C[AT][AT]GG
TaqI 13 TCGA T^CGA
Tru9I 236 TTAA T^TAA
Tsp509I 155 AATT ^AATT
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 13 TCGA T^CGA
Van91I 337 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 214 AGCT AG^CT
302 AGCT
BstNI 72 CCAGG CC^[AT]GG
270 CCAGG
BstYI 179 AGATCC [GA]^GATC[CT]
273 GGATCC
Cfr13I 54 GGTCC G^G[ACGT]CC
346 GGGCC
DdeI 38 CTGAG C^T[ACGT]AG
278 CTGAG
DsaI 167 CCGTGG C^C[GA][CT]GG
342 CCATGG
EaeI 170 TGGCCA [CT]^GGCC[GA]
368 TGGCCG
EcoRII 72 CCAGG ^CC[AT]GG
270 CCAGG
HaeII 34 AGCGCT [GA]GCGC^[CT]
309 GGCGCT
HapII 22 CCGG C^CGG
88 CCGG
HpaII 22 CCGG C^CGG
88 CCGG
MflI 179 AGATCC [GA]^GATC[CT]
273 GGATCC
MspI 22 CCGG C^CGG
88 CCGG
MvaI 72 CCAGG CC^[AT]GG
270 CCAGG
Sau96I 54 GGTCC G^G[ACGT]CC
346 GGGCC
SmlI 30 CTTGAG C^T[CT][GA]AG
235 CTTAAG
XhoII 179 AGATCC [GA]^GATC[CT]
273 GGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AlwI BbvI BsaJI BsiYI
BslI BssKI Cac8I CfoI DpnII
Fnu4HI HaeIII HhaI HinP1I ItaI
MboI MnlI MwoI NdeII NlaIII
Sau3AI ScrFI TseI