Restriction map for
pFN21AE3980
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsiWI Bsp1407I BspDI BspMI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I HindIII HpaI
KpnI KspI MluI MseI MvnI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflIII 1280 ACATGT A^C[GA][CT]GT
AvaI 1504 CCCGAG C^[CT]CG[GA]G
AviII 167 TGCGCA TGC^GCA
BalI 1013 TGGCCA TGG^CCA
BanI 30 GGCGCC G^G[CT][GA]CC
BbeI 30 GGCGCC GGCGC^C
BbsI 682 GAAGAC GAAGAC(2/6)
BcgI 524 GCATCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 131 TGATCA T^GATCA
BfaI 857 CTAG C^TAG
BglI 388 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 682 GAAGAC GAAGAC(2/6)
BsaBI 132 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 946 GTGCAG GTGCAG(16/14)
BsiEI 258 CGACCG CG[GA][CT]^CG
BsmI 1571 GAATGC GAATGC(1/-1)
BsoBI 1504 CCCGAG C^[CT]CG[GA]G
BspHI 884 TCATGA T^CATGA
BspLU11I 1280 ACATGT A^CATGT
BsrBI 121 GAGCGG CCGCTC(-3/-3)
BsrDI 1347 CATTGC GCAATG(2/0)
Bst1107I 1428 GTATAC GTA^TAC
BstZ17I 1428 GTATAC GTA^TAC
EcoNI 1112 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1076 CCAAGG C^C[AT][AT]GG
FbaI 131 TGATCA T^GATCA
FseI 512 GGCCGGCC GGCCGG^CC
FspI 167 TGCGCA TGC^GCA
HincII 328 GTCAAC GT[CT]^[GA]AC
HindII 328 GTCAAC GT[CT]^[GA]AC
KasI 30 GGCGCC G^GCGCC
MaeI 857 CTAG C^TAG
MamI 132 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 163 CAATTG C^AATTG
MluNI 1013 TGGCCA TGG^CCA
MscI 1013 TGGCCA TGG^CCA
MslI 1290 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 163 CAATTG C^AATTG
NarI 30 GGCGCC GG^CGCC
NspI 1280 ACATGT [GA]CATG^[CT]
PflMI 967 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1337 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1084 CTGCAG CTGCA^G
PvuII 373 CAGCTG CAG^CTG
RcaI 884 TCATGA T^CATGA
SfiI 387 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 30 GGCGCC GGC^GCC
StuI 1109 AGGCCT AGG^CCT
StyI 1076 CCAAGG C^C[AT][AT]GG
Tsp45I 1438 GTCAC ^GT[GC]AC
Tsp509I 164 AATT ^AATT
Van91I 967 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1180 GTCTAC GT^[AC][GT]AC
1428 GTATAC
AccIII 186 TCCGGA T^CCGGA
875 TCCGGA
AcyI 30 GGCGCC G[GA]^CG[CT]C
280 GACGCC
AlwI 360 GATCC GGATC(4/5)
1089 GATCC
AlwNI 1087 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1190 CAGAGGCTG
AocI 237 CCTGAGG CC^T[ACGT]AGG
1464 CCTGAGG
AspHI 504 GAGCTC G[AT]GC[AT]^C
829 GAGCTC
BsaHI 30 GGCGCC G[GA]^CG[CT]C
280 GACGCC
BsaWI 186 TCCGGA [AT]^CCGG[AT]
875 TCCGGA
BseAI 186 TCCGGA T^CCGGA
875 TCCGGA
BsiHKAI 504 GAGCTC G[AT]GC[AT]^C
829 GAGCTC
BspEI 186 TCCGGA T^CCGGA
875 TCCGGA
BstXI 1004 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCATGACTGTGG
BstYI 359 AGATCC [GA]^GATC[CT]
1088 AGATCC
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
1464 CCTGAGG
DraII 386 AGGCCCT [GA]G^G[ACGT]CC[CT]
1337 GGGTCCT
DsaI 45 CCGTGG C^C[GA][CT]GG
1262 CCACGG
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
1464 CCTGAGG
EcoO109I 386 AGGCCCT [GA]G^G[ACGT]CC[CT]
1337 GGGTCCT
HaeII 30 GGCGCC [GA]GCGC^[CT]
470 AGCGCC
HgaI 280 GACGC GACGC(5/10)
801 GACGC
Hin1I 30 GGCGCC G[GA]^CG[CT]C
280 GACGCC
MaeII 332 ACGT A^CGT
1436 ACGT
MflI 359 AGATCC [GA]^GATC[CT]
1088 AGATCC
MroI 186 TCCGGA T^CCGGA
875 TCCGGA
MspA1I 373 CAGCTG C[AC]G^C[GT]G
448 CCGCTG
SacI 504 GAGCTC GAGCT^C
829 GAGCTC
ScaI 248 AGTACT AGT^ACT
754 AGTACT
SfcI 111 CTGTAG C^T[GA][CT]AG
1084 CTGCAG
TfiI 688 GATTC G^A[AT]TC
1207 GATTC
XcmI 1115 CCATGAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1434 CCACGTCACTTGTGG
XhoII 359 AGATCC [GA]^GATC[CT]
1088 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TspRI TthHB8I