Restriction map for
pFN21AE5415
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DpnII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 164 TCCGGA T^CCGGA
AvaII 912 GGTCC G^G[AT]CC
BsaJI 999 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 164 TCCGGA [AT]^CCGG[AT]
BseAI 164 TCCGGA T^CCGGA
BseRI 416 CTCCTC GAGGAG(10/8)
BsmFI 913 GTCCC GGGAC(10/14)
Bsp1407I 253 TGTACA T^GTACA
BspEI 164 TCCGGA T^CCGGA
BsrGI 253 TGTACA T^GTACA
BssKI 604 CCTGG ^CC[ACGT]GG
BssSI 1019 CACGAG CACGAG(-5/-1)
BstNI 604 CCTGG CC^[AT]GG
Cfr13I 912 GGTCC G^G[ACGT]CC
DraI 93 TTTAAA TTT^AAA
DraII 911 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 602 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 911 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 604 CCTGG ^CC[AT]GG
EcoT14I 999 CCTTGG C^C[AT][AT]GG
HaeIII 8 GGCC GG^CC
HapII 165 CCGG C^CGG
HpaII 165 CCGG C^CGG
MroI 164 TCCGGA T^CCGGA
MspI 165 CCGG C^CGG
MvaI 604 CCTGG CC^[AT]GG
NlaIII 382 CATG CATG^
PpuMI 911 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 527 CTGCAG CTGCA^G
Sau96I 912 GGTCC G^G[ACGT]CC
ScrFI 604 CCTGG CC^[ACGT]GG
SexAI 603 ACCTGGT A^CC[AT]GGT
SfcI 527 CTGCAG C^T[GA][CT]AG
SmlI 359 CTCAAG C^T[CT][GA]AG
SspBI 253 TGTACA T^GTACA
StyI 999 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbsI 658 GAAGAC GAAGAC(2/6)
843 GTCTTC
BpmI 791 CTCCAG CTGGAG(16/14)
929 CTGGAG
BpuAI 658 GAAGAC GAAGAC(2/6)
843 GTCTTC
BsgI 242 GTGCAG GTGCAG(16/14)
349 GTGCAG
BsiYI 915 CCCACCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
954 CCCACTGGTGG
BslI 915 CCCACCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
954 CCCACTGGTGG
BstF5I 1036 CATCC GGATG(2/0)
621 GGATG
FokI 1036 CATCC GGATG(9/13)
621 GGATG
MaeIII 337 GTCAC ^GT[ACGT]AC
772 GTGAC
MslI 342 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1036 CATCCAAATG
NlaIV 911 GGGTCC GG[ACGT]^[ACGT]CC
912 GGTCCC
PvuII 794 CAGCTG CAG^CTG
887 CAGCTG
Tsp45I 337 GTCAC ^GT[GC]AC
772 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsmAI BsrI DdeI Fnu4HI HinfI
HphI ItaI MaeI MboII MnlI
MseI MspA1I MwoI PleI RsaI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI