Restriction map for
pFN21AE6466
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BlpI Bpu1102I
BsaAI BsaHI BsgI BsiEI BsiWI
BsmI BspDI BspHI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MseI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI SacII SalI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 391 GTCTAC GT^[AC][GT]AC
AccII 1261 CGCG CG^CG
AccIII 1244 TCCGGA T^CCGGA
AflIII 1226 ACATGT A^C[GA][CT]GT
AhdI 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 102 GGATC GGATC(4/5)
AlwNI 1073 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 841 CCTCAGG CC^T[ACGT]AGG
ApaI 1789 GGGCCC GGGCC^C
AspEI 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1264 GAGCTC G[AT]GC[AT]^C
AvrII 1170 CCTAGG C^CTAGG
BalI 1529 TGGCCA TGG^CCA
BciVI 1603 GGATAC GTATCC(6/5)
BclI 761 TGATCA T^GATCA
BfaI 1171 CTAG C^TAG
BlnI 1170 CCTAGG C^CTAGG
BsaBI 1332 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1244 TCCGGA [AT]^CCGG[AT]
BseAI 1244 TCCGGA T^CCGGA
BsiHKAI 1264 GAGCTC G[AT]GC[AT]^C
BsmBI 579 GAGACG CGTCTC(1/5)
Bsp1407I 1334 TGTACA T^GTACA
BspEI 1244 TCCGGA T^CCGGA
BspLU11I 1226 ACATGT A^CATGT
BspMI 466 ACCTGC ACCTGC(4/8)
BsrBI 1251 GAGCGG CCGCTC(-3/-3)
BsrGI 1334 TGTACA T^GTACA
BstAPI 1041 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1317 GGTCACC G^GT[ACGT]ACC
BstPI 1317 GGTCACC G^GT[ACGT]ACC
BstUI 1261 CGCG CG^CG
Bsu36I 841 CCTCAGG CC^T[ACGT]AGG
CpoI 1433 CGGACCG CG^G[AT]CCG
Eam1105I 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 841 CCTCAGG CC^T[ACGT]AGG
EcoNI 339 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1317 GGTCACC G^GT[ACGT]ACC
EcoRI 568 GAATTC G^AATTC
FbaI 761 TGATCA T^GATCA
MaeI 1171 CTAG C^TAG
MamI 1332 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1529 TGGCCA TGG^CCA
MroI 1244 TCCGGA T^CCGGA
MscI 1529 TGGCCA TGG^CCA
MvnI 1261 CGCG CG^CG
NaeI 523 GCCGGC GCC^GGC
NdeI 1374 CATATG CA^TATG
NgoMIV 523 GCCGGC G^CCGGC
NspI 1226 ACATGT [GA]CATG^[CT]
PshAI 1726 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1433 CGGACCG CG^G[AT]CCG
SacI 1264 GAGCTC GAGCT^C
SexAI 92 ACCAGGT A^CC[AT]GGT
SspBI 1334 TGTACA T^GTACA
TaqI 374 TCGA T^CGA
TthHB8I 374 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 546 GAATTT [GA]^AATT[CT]
568 GAATTC
ApoI 546 GAATTT [GA]^AATT[CT]
568 GAATTC
BsaI 1831 GAGACC GGTCTC(1/5)
42 GGTCTC
BseRI 1267 CTCCTC GAGGAG(10/8)
58 GAGGAG
BsrDI 493 CATTGC GCAATG(2/0)
170 GCAATG
BsrFI 523 GCCGGC [GA]^CCGG[CT]
1472 ACCGGC
CfoI 627 GCGC GCG^C
1260 GCGC
Cfr10I 523 GCCGGC [GA]^CCGG[CT]
1472 ACCGGC
DsaI 1477 CCATGG C^C[GA][CT]GG
1637 CCATGG
HgaI 1701 GCGTC GACGC(5/10)
1707 GCGTC
HhaI 627 GCGC GCG^C
1260 GCGC
HinP1I 627 GCGC G^CGC
1260 GCGC
MaeII 730 ACGT A^CGT
1580 ACGT
MslI 258 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1221 CATAGACATG
MspA1I 1132 CAGCTG C[AC]G^C[GT]G
1345 CAGCTG
NcoI 1477 CCATGG C^CATGG
1637 CCATGG
PflMI 609 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1610 CCATTGGCTGG
PvuII 1132 CAGCTG CAG^CTG
1345 CAGCTG
SapI 1248 GAAGAGC GCTCTTC(1/4)
1503 GCTCTTC
Tsp509I 547 AATT ^AATT
569 AATT
Van91I 609 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1610 CCATTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BglII BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI Tsp45I
TspRI XcmI XhoII