Restriction map for
pFN21AE6631
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI AlwNI Aor51HI
AscI Asp700I AspEI AspI AvrII
BalI BbeI BbrPI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CelII Cfr10I ClaI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoT22I
FauI FbaI FseI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 14 GGTACC G^GTACC
AccI 482 GTAGAC GT^[AC][GT]AC
Alw44I 168 GTGCAC G^TGCAC
AocI 162 CCTCAGG CC^T[ACGT]AGG
ApaI 330 GGGCCC GGGCC^C
ApaLI 168 GTGCAC G^TGCAC
AseI 439 ATTAAT AT^TAAT
AsnI 439 ATTAAT AT^TAAT
Asp718I 14 GGTACC G^GTACC
AviII 92 TGCGCA TGC^GCA
BamHI 252 GGATCC G^GATCC
BanII 330 GGGCCC G[GA]GC[CT]^C
BbsI 544 GAAGAC GAAGAC(2/6)
BcgI 597 CGAGTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 377 CTAG C^TAG
BpuAI 544 GAAGAC GAAGAC(2/6)
BssSI 40 CTCGTG CACGAG(-5/-1)
Bsu36I 162 CCTCAGG CC^T[ACGT]AGG
Cac8I 916 GCCAGC GC[ACGT]^[ACGT]GC
CpoI 137 CGGTCCG CG^G[AT]CCG
DrdI 178 GACAACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 620 TGGCCG [CT]^GGCC[GA]
Eco81I 162 CCTCAGG CC^T[ACGT]AGG
EcoRI 754 GAATTC G^AATTC
EcoRV 430 GATATC GAT^ATC
EcoT14I 683 CCAAGG C^C[AT][AT]GG
FspI 92 TGCGCA TGC^GCA
HaeII 154 AGCGCC [GA]GCGC^[CT]
KpnI 14 GGTACC GGTAC^C
MaeI 377 CTAG C^TAG
MseI 440 TTAA T^TAA
MspA1I 96 CAGCGG C[AC]G^C[GT]G
MwoI 461 GCCTCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 306 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 439 ATTAAT AT^TAAT
PstI 721 CTGCAG CTGCA^G
RsrII 137 CGGTCCG CG^G[AT]CCG
SfcI 721 CTGCAG C^T[GA][CT]AG
SspI 454 AATATT AAT^ATT
StyI 683 CCAAGG C^C[AT][AT]GG
TaqI 299 TCGA T^CGA
Tru9I 440 TTAA T^TAA
TthHB8I 299 TCGA T^CGA
Van91I 306 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 259 CGCG CG^CG
624 CGCG
AcsI 560 AAATTC [GA]^AATT[CT]
754 GAATTC
AluI 120 AGCT AG^CT
899 AGCT
ApoI 560 AAATTC [GA]^AATT[CT]
754 GAATTC
AspHI 168 GTGCAC G[AT]GC[AT]^C
943 GTGCTC
AvaI 191 CCCGGG C^[CT]CG[GA]G
575 CCCGGG
BanI 14 GGTACC G^G[CT][GA]CC
245 GGTGCC
BbvCI 734 CCTCAGC CCTCAGC(-5/-2)
746 CCTCAGC
BsgI 349 GTGCAG GTGCAG(16/14)
600 GTGCAG
BsiHKAI 168 GTGCAC G[AT]GC[AT]^C
943 GTGCTC
BsoBI 191 CCCGGG C^[CT]CG[GA]G
575 CCCGGG
BspMI 340 ACCTGC ACCTGC(4/8)
466 ACCTGC
BsrI 478 ACTGG ACTGG(1/-1)
759 CCAGT
BstEII 186 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
BstPI 186 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
BstUI 259 CGCG CG^CG
624 CGCG
BstYI 252 GGATCC [GA]^GATC[CT]
302 AGATCC
DpnII 253 GATC ^GATC
303 GATC
DraII 329 AGGGCCC [GA]G^G[ACGT]CC[CT]
330 GGGCCCT
DsaI 629 CCGTGG C^C[GA][CT]GG
701 CCGTGG
EcoO109I 329 AGGGCCC [GA]G^G[ACGT]CC[CT]
330 GGGCCCT
EcoO65I 186 GGTCACC G^GT[ACGT]ACC
648 GGTCACC
MboI 253 GATC ^GATC
303 GATC
MflI 252 GGATCC [GA]^GATC[CT]
302 AGATCC
MvnI 259 CGCG CG^CG
624 CGCG
NdeII 253 GATC ^GATC
303 GATC
NlaIII 862 CATG CATG^
924 CATG
Sau3AI 253 GATC ^GATC
303 GATC
SmaI 191 CCCGGG CCC^GGG
575 CCCGGG
XhoII 252 GGATCC [GA]^GATC[CT]
302 AGATCC
XmaCI 191 CCCGGG C^CCGGG
575 CCCGGG
XmaI 191 CCCGGG C^CCGGG
575 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AlwI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstXI CfoI Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspI MvaI NciI NlaIV RsaI
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I TspRI