Restriction map for
pFN21AE6764
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI Aor51HI ApoI AseI AsnI
AspI AvrII BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI MamI MfeI MluI
MunI NcoI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SfuI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1006 GGTACC G^GTACC
AhdI 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2838 CCTGAGG CC^T[ACGT]AGG
AscI 623 GGCGCGCC GG^CGCGCC
Asp700I 1998 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1006 GGTACC G^GTACC
AspEI 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 866 TGCGCA TGC^GCA
BamHI 1566 GGATCC G^GATCC
BbrPI 1093 CACGTG CAC^GTG
BbvCI 2475 CCTCAGC CCTCAGC(-5/-2)
BfaI 425 CTAG C^TAG
BglI 2765 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 503 GAGACC GGTCTC(1/5)
BsmBI 661 GAGACG CGTCTC(1/5)
BspLU11I 1591 ACATGT A^CATGT
BsrDI 848 GCAATG GCAATG(2/0)
BssSI 1081 CACGAG CACGAG(-5/-1)
Bsu36I 2838 CCTGAGG CC^T[ACGT]AGG
DrdI 2827 GACTTCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2838 CCTGAGG CC^T[ACGT]AGG
EcoNI 1296 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2077 ATGCAT ATGCA^T
FspI 866 TGCGCA TGC^GCA
KpnI 1006 GGTACC GGTAC^C
MaeI 425 CTAG C^TAG
MseI 104 TTAA T^TAA
NheI 424 GCTAGC G^CTAGC
NsiI 2077 ATGCAT ATGCA^T
PmaCI 1093 CACGTG CAC^GTG
PmlI 1093 CACGTG CAC^GTG
SacI 2692 GAGCTC GAGCT^C
SapI 281 GCTCTTC GCTCTTC(1/4)
SbfI 2304 CCTGCAGG CCTGCA^GG
SfiI 2764 GGCCACGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 431 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2304 CCTGCAGG CCTGCA^GG
TfiI 2003 GATTC G^A[AT]TC
Tru9I 104 TTAA T^TAA
Tsp509I 2089 AATT ^AATT
XmnI 1998 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 499 TCCGGA T^CCGGA
1723 TCCGGA
AflIII 1094 ACGTGT A^C[GA][CT]GT
1591 ACATGT
Alw44I 1407 GTGCAC G^TGCAC
1626 GTGCAC
ApaI 557 GGGCCC GGGCC^C
832 GGGCCC
ApaLI 1407 GTGCAC G^TGCAC
1626 GTGCAC
BalI 1613 TGGCCA TGG^CCA
2099 TGGCCA
BlpI 1715 GCTCAGC GC^T[ACGT]AGC
2694 GCTCAGC
Bpu1102I 1715 GCTCAGC GC^T[ACGT]AGC
2694 GCTCAGC
BsaAI 655 TACGTG [CT]AC^GT[GA]
1093 CACGTG
BsaWI 499 TCCGGA [AT]^CCGG[AT]
1723 TCCGGA
BseAI 499 TCCGGA T^CCGGA
1723 TCCGGA
BseRI 1132 GAGGAG GAGGAG(10/8)
1741 GAGGAG
BspEI 499 TCCGGA T^CCGGA
1723 TCCGGA
BsrBI 64 GAGCGG CCGCTC(-3/-3)
2239 GAGCGG
BssHII 428 GCGCGC G^CGCGC
624 GCGCGC
BstEII 840 GGTCACC G^GT[ACGT]ACC
2705 GGTCACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
2705 GGTCACC
BstYI 1305 GGATCT [GA]^GATC[CT]
1566 GGATCC
CelII 1715 GCTCAGC GC^T[ACGT]AGC
2694 GCTCAGC
DraIII 1537 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1914 CACGCTGTG
EagI 108 CGGCCG C^GGCCG
2727 CGGCCG
EclXI 108 CGGCCG C^GGCCG
2727 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
2727 CGGCCG
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
2705 GGTCACC
MflI 1305 GGATCT [GA]^GATC[CT]
1566 GGATCC
MluNI 1613 TGGCCA TGG^CCA
2099 TGGCCA
MroI 499 TCCGGA T^CCGGA
1723 TCCGGA
MscI 1613 TGGCCA TGG^CCA
2099 TGGCCA
SexAI 2246 ACCTGGT A^CC[AT]GGT
2489 ACCAGGT
SmlI 2118 CTCAAG C^T[CT][GA]AG
2752 CTCAAG
XcmI 239 CCACTGAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1421 CCAAGGGACAGATGG
XhoII 1305 GGATCT [GA]^GATC[CT]
1566 GGATCC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XmaCI XmaI