Restriction map for
pFN21AE5557
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbrPI BbsI BcgI BciVI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 21 CGCG CG^CG
AcsI 293 GAATTC [GA]^AATT[CT]
AcyI 6 GGCGCC G[GA]^CG[CT]C
AlwNI 892 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 27 GGGCCC GGGCC^C
ApoI 293 GAATTC [GA]^AATT[CT]
AspI 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 717 TGCGCA TGC^GCA
BalI 417 TGGCCA TGG^CCA
BamHI 35 GGATCC G^GATCC
BbeI 6 GGCGCC GGCGC^C
BbvCI 584 CCTCAGC CCTCAGC(-5/-2)
BclI 855 TGATCA T^GATCA
BfaI 928 CTAG C^TAG
BglI 109 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 747 GCTCAGC GC^T[ACGT]AGC
BpmI 781 CTCCAG CTGGAG(16/14)
Bpu1102I 747 GCTCAGC GC^T[ACGT]AGC
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsaI 340 GGTCTC GGTCTC(1/5)
BseRI 762 CTCCTC GAGGAG(10/8)
BsmBI 78 CGTCTC CGTCTC(1/5)
BspMI 962 ACCTGC ACCTGC(4/8)
BssSI 772 CTCGTG CACGAG(-5/-1)
BstUI 21 CGCG CG^CG
BstXI 1112 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 35 GGATCC [GA]^GATC[CT]
CelII 747 GCTCAGC GC^T[ACGT]AGC
DraII 27 GGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 67 CTCTTC CTCTTC(1/4)
EcoO109I 27 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 293 GAATTC G^AATTC
FbaI 855 TGATCA T^GATCA
FspI 717 TGCGCA TGC^GCA
HaeII 6 GGCGCC [GA]GCGC^[CT]
HgaI 22 GCGTC GACGC(5/10)
Hin1I 6 GGCGCC G[GA]^CG[CT]C
KasI 6 GGCGCC G^GCGCC
Ksp632I 67 CTCTTC CTCTTC(1/4)
MaeI 928 CTAG C^TAG
MaeII 266 ACGT A^CGT
MaeIII 730 GTAAC ^GT[ACGT]AC
MflI 35 GGATCC [GA]^GATC[CT]
MluNI 417 TGGCCA TGG^CCA
MscI 417 TGGCCA TGG^CCA
MslI 561 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 21 CGCG CG^CG
NarI 6 GGCGCC GG^CGCC
NspI 570 GCATGT [GA]CATG^[CT]
PflFI 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 247 CTGCAG CTGCA^G
SacI 778 GAGCTC GAGCT^C
SexAI 740 ACCAGGT A^CC[AT]GGT
SfcI 247 CTGCAG C^T[GA][CT]AG
SfoI 6 GGCGCC GGC^GCC
Tsp509I 294 AATT ^AATT
Tth111I 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 35 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 287 CCCGAG C^[CT]CG[GA]G
663 CCCGAG
AvaII 366 GGTCC G^G[AT]CC
998 GGTCC
BanI 6 GGCGCC G^G[CT][GA]CC
907 GGCACC
BsgI 964 CTGCAC GTGCAG(16/14)
1057 CTGCAC
BsmI 714 GAATGC GAATGC(1/-1)
1050 GCATTC
BsoBI 287 CCCGAG C^[CT]CG[GA]G
663 CCCGAG
BsrFI 186 GCCGGT [GA]^CCGG[CT]
449 ACCGGC
BstAPI 1050 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1059 GCACCGCCTGC
BstF5I 603 CATCC GGATG(2/0)
951 CATCC
Cfr10I 186 GCCGGT [GA]^CCGG[CT]
449 ACCGGC
EcoT14I 51 CCAAGG C^C[AT][AT]GG
849 CCTTGG
FauI 944 CCCGC CCCGC(4/6)
59 GCGGG
FokI 603 CATCC GGATG(9/13)
951 CATCC
PflMI 447 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
844 CCAATCCTTGG
PvuII 244 CAGCTG CAG^CTG
976 CAGCTG
ScaI 551 AGTACT AGT^ACT
863 AGTACT
StyI 51 CCAAGG C^C[AT][AT]GG
849 CCTTGG
TaqI 813 TCGA T^CGA
937 TCGA
TthHB8I 813 TCGA T^CGA
937 TCGA
Van91I 447 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
844 CCAATCCTTGG
XcmI 467 CCACAGGTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
783 CCAGCCCATCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI