Restriction map for
pFN21AE6537
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I KspI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcyI 73 GGCGCC G[GA]^CG[CT]C
AluI 189 AGCT AG^CT
AlwI 67 GATCC GGATC(4/5)
BanI 73 GGCGCC G^G[CT][GA]CC
BbeI 73 GGCGCC GGCGC^C
BbvI 8 GCTGC GCAGC(8/12)
BfaI 49 CTAG C^TAG
BsaHI 73 GGCGCC G[GA]^CG[CT]C
BsgI 9 CTGCAC GTGCAG(16/14)
BsiYI 166 CCGGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 166 CCGGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 230 GAGAC GTCTC(1/5)
BsrBI 141 GAGCGG CCGCTC(-3/-3)
BstF5I 54 CATCC GGATG(2/0)
DpnII 67 GATC ^GATC
FokI 54 CATCC GGATG(9/13)
HaeII 73 GGCGCC [GA]GCGC^[CT]
Hin1I 73 GGCGCC G[GA]^CG[CT]C
KasI 73 GGCGCC G^GCGCC
MaeI 49 CTAG C^TAG
MboI 67 GATC ^GATC
MnlI 206 GAGG CCTC(7/6)
NarI 73 GGCGCC GG^CGCC
NdeII 67 GATC ^GATC
NlaIII 120 CATG CATG^
NotI 82 GCGGCCGC GC^GGCCGC
Sau3AI 67 GATC ^GATC
SfiI 154 GGCCGGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 73 GGCGCC GGC^GCC
TseI 8 GCTGC G^C[AT]GC
XbaI 48 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 162 GGGCCC GGGCC^C
168 GGGCCC
AvaI 165 CCCGGG C^[CT]CG[GA]G
200 CCCGGG
BanII 162 GGGCCC G[GA]GC[CT]^C
168 GGGCCC
BglI 76 GCCAACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
155 GCCGGAAGGGC
BmyI 162 GGGCCC G[AGT]GC[ACT]^C
168 GGGCCC
BsiEI 83 CGGCCG CG[GA][CT]^CG
153 CGGCCG
BsoBI 165 CCCGGG C^[CT]CG[GA]G
200 CCCGGG
Bsp1286I 162 GGGCCC G[AGT]GC[ACT]^C
168 GGGCCC
Cac8I 22 GCCTGC GC[ACGT]^[ACGT]GC
211 GCGGGC
EaeI 83 CGGCCG [CT]^GGCC[GA]
153 CGGCCG
EagI 83 CGGCCG C^GGCCG
153 CGGCCG
EclXI 83 CGGCCG C^GGCCG
153 CGGCCG
Eco52I 83 CGGCCG C^GGCCG
153 CGGCCG
FauI 57 CCCGC CCCGC(4/6)
211 GCGGG
MboII 129 GAAGA GAAGA(8/7)
132 GAAGA
MspA1I 58 CCGCTG C[AC]G^C[GT]G
225 CAGCGG
SmaI 165 CCCGGG CCC^GGG
200 CCCGGG
XmaCI 165 CCCGGG C^CCGGG
200 CCCGGG
XmaI 165 CCCGGG C^CCGGG
200 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI BcnI BsaJI BssKI
BstNI BstUI CfoI Cfr13I DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI