##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvaI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       TspRI       Tth111I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccII          73  CGCG                 CG^CG
AciI          198  CCGC                 CCGC(-3/-1)
AcsI           32  GAATTC               [GA]^AATT[CT]
ApoI           32  GAATTC               [GA]^AATT[CT]
BcnI          375  CCGGG                CC^[GC]GG
BfaI          139  CTAG                 C^TAG
BsaJI         375  CCGGGG               C^C[ACGT][ACGT]GG
BsaWI          93  ACCGGA               [AT]^CCGG[AT]
BsmFI         378  GGGAC                GGGAC(10/14)
BsmI          217  GCATTC               GAATGC(1/-1)
BssKI         375  CCGGG                ^CC[ACGT]GG
BstUI          73  CGCG                 CG^CG
BstXI         169  CCAGCAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI          32  GAATTC               G^AATTC
EcoRV         273  GATATC               GAT^ATC
EcoT22I       215  ATGCAT               ATGCA^T
HphI          302  TCACC                GGTGA(8/7)
MaeI          139  CTAG                 C^TAG
MaeIII        301  GTCAC                ^GT[ACGT]AC
MvnI           73  CGCG                 CG^CG
MwoI          109  GCTTTTGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          375  CCGGG                CC^[GC]GG
NlaIII        214  CATG                 CATG^
NsiI          215  ATGCAT               ATGCA^T
PshAI         294  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScrFI         375  CCGGG                CC^[ACGT]GG
Tsp45I        301  GTCAC                ^GT[GC]AC
Tsp509I        33  AATT                 ^AATT
XbaI          138  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          108  AGCT                 AG^CT
              366  AGCT                
AlwI          166  GATCC                GGATC(4/5)
              250  GATCC               
BbsI          193  GAAGAC               GAAGAC(2/6)
              422  GTCTTC              
BpuAI         193  GAAGAC               GAAGAC(2/6)
              422  GTCTTC              
BseRI         223  GAGGAG               GAGGAG(10/8)
              394  GAGGAG              
BsmAI         292  GAGAC                GTCTC(1/5)
              267  GTCTC               
DpnII         166  GATC                 ^GATC
              250  GATC                
HapII          94  CCGG                 C^CGG
              375  CCGG                
HpaII          94  CCGG                 C^CGG
              375  CCGG                
MboI          166  GATC                 ^GATC
              250  GATC                
MspI           94  CCGG                 C^CGG
              375  CCGG                
NdeII         166  GATC                 ^GATC
              250  GATC                
PleI          313  GACTC                GAGTC(4/5)
              265  GAGTC               
Sau3AI        166  GATC                 ^GATC
              250  GATC                
TaqI            4  TCGA                 T^CGA
              221  TCGA                
TthHB8I         4  TCGA                 T^CGA
              221  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BbvI        DdeI        EarI        Fnu4HI      HinfI       
ItaI        Ksp632I     MboII       MnlI        TfiI        
TseI