Restriction map for
pFN21AE5425
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 886 GACGTC GACGT^C
AciI 363 CCGC CCGC(-3/-1)
AcsI 12 GAATTC [GA]^AATT[CT]
AcyI 886 GACGTC G[GA]^CG[CT]C
AflIII 530 ACATGT A^C[GA][CT]GT
AlwI 72 GATCC GGATC(4/5)
AlwNI 714 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApoI 12 GAATTC [GA]^AATT[CT]
AseI 861 ATTAAT AT^TAAT
AsnI 861 ATTAAT AT^TAAT
AvaII 701 GGTCC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BanI 843 GGTGCC G^G[CT][GA]CC
BbsI 819 GTCTTC GAAGAC(2/6)
BcnI 61 CCGGG CC^[GC]GG
BfaI 94 CTAG C^TAG
BpmI 666 CTCCAG CTGGAG(16/14)
BpuAI 819 GTCTTC GAAGAC(2/6)
BsaAI 103 TACGTG [CT]AC^GT[GA]
BsaHI 886 GACGTC G[GA]^CG[CT]C
Bsp1407I 392 TGTACA T^GTACA
BspLU11I 530 ACATGT A^CATGT
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 392 TGTACA T^GTACA
BsrI 221 CCAGT ACTGG(1/-1)
BstEII 399 GGTCACC G^GT[ACGT]ACC
BstPI 399 GGTCACC G^GT[ACGT]ACC
BstYI 71 AGATCC [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
DpnII 72 GATC ^GATC
DraIII 477 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 371 TGGCCA [CT]^GGCC[GA]
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO65I 399 GGTCACC G^GT[ACGT]ACC
EcoRI 12 GAATTC G^AATTC
EcoT14I 467 CCATGG C^C[AT][AT]GG
EcoT22I 923 ATGCAT ATGCA^T
HapII 61 CCGG C^CGG
Hin1I 886 GACGTC G[GA]^CG[CT]C
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
HpaII 61 CCGG C^CGG
MaeI 94 CTAG C^TAG
MboI 72 GATC ^GATC
MflI 71 AGATCC [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspI 61 CCGG C^CGG
NciI 61 CCGGG CC^[GC]GG
NcoI 467 CCATGG C^CATGG
NdeII 72 GATC ^GATC
NsiI 923 ATGCAT ATGCA^T
NspI 530 ACATGT [GA]CATG^[CT]
PshBI 861 ATTAAT AT^TAAT
PstI 720 CTGCAG CTGCA^G
SapI 300 GCTCTTC GCTCTTC(1/4)
Sau3AI 72 GATC ^GATC
SfcI 720 CTGCAG C^T[GA][CT]AG
SmlI 553 CTTGAG C^T[CT][GA]AG
SspBI 392 TGTACA T^GTACA
StyI 467 CCATGG C^C[AT][AT]GG
TfiI 685 GATTC G^A[AT]TC
XhoII 71 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbvI 722 GCAGC GCAGC(8/12)
4 GCTGC
BmyI 625 GTGCCC G[AGT]GC[ACT]^C
844 GTGCCC
BsmAI 325 GTCTC GTCTC(1/5)
408 GTCTC
BsmI 766 GCATTC GAATGC(1/-1)
925 GCATTC
Bsp1286I 625 GTGCCC G[AGT]GC[ACT]^C
844 GTGCCC
BspMI 718 ACCTGC ACCTGC(4/8)
698 GCAGGT
DsaI 467 CCATGG C^C[GA][CT]GG
689 CCACGG
EarI 79 CTCTTC CTCTTC(1/4)
301 CTCTTC
Fnu4HI 4 GCTGC GC^[ACGT]GC
722 GCAGC
HphI 401 TCACC GGTGA(8/7)
635 TCACC
ItaI 4 GCTGC GC^[ACGT]GC
722 GCAGC
Ksp632I 79 CTCTTC CTCTTC(1/4)
301 CTCTTC
MaeII 104 ACGT A^CGT
887 ACGT
MwoI 472 GCCCACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
716 GCACCTGCAGC
SfaNI 272 GCATC GCATC(5/9)
671 GCATC
TseI 4 GCTGC G^C[AT]GC
722 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII FokI HaeIII HinfI
MaeIII MboII MnlI MseI MslI
MvaI NlaIII NlaIV PleI RsaI
Sau96I ScrFI Tru9I Tsp45I Tsp509I
TspRI