Restriction map for
pFN21AB7735
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII AccII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MslI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1277 GGTACC G^GTACC
AccI 1051 GTCTAC GT^[AC][GT]AC
AccIII 217 TCCGGA T^CCGGA
AcyI 995 GACGCC G[GA]^CG[CT]C
AflIII 722 ACATGT A^C[GA][CT]GT
AlwNI 325 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1076 CCTCAGG CC^T[ACGT]AGG
ApaI 1089 GGGCCC GGGCC^C
Asp718I 1277 GGTACC G^GTACC
AspHI 795 GAGCAC G[AT]GC[AT]^C
AvaI 562 CTCGAG C^[CT]CG[GA]G
BalI 1028 TGGCCA TGG^CCA
BamHI 469 GGATCC G^GATCC
BcgI 1012 CGAATCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 319 GTATCC GTATCC(6/5)
BfaI 115 CTAG C^TAG
BsaHI 995 GACGCC G[GA]^CG[CT]C
BsaWI 217 TCCGGA [AT]^CCGG[AT]
BseAI 217 TCCGGA T^CCGGA
BsiHKAI 795 GAGCAC G[AT]GC[AT]^C
BsoBI 562 CTCGAG C^[CT]CG[GA]G
BspEI 217 TCCGGA T^CCGGA
BspLU11I 722 ACATGT A^CATGT
BspMI 204 ACCTGC ACCTGC(4/8)
BsrBI 277 CCGCTC CCGCTC(-3/-3)
Bsu36I 1076 CCTCAGG CC^T[ACGT]AGG
CfoI 603 GCGC GCG^C
Eco81I 1076 CCTCAGG CC^T[ACGT]AGG
EcoNI 1180 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 28 GATATC GAT^ATC
EcoT14I 956 CCATGG C^C[AT][AT]GG
HgaI 995 GACGC GACGC(5/10)
HhaI 603 GCGC GCG^C
Hin1I 995 GACGCC G[GA]^CG[CT]C
HinP1I 603 GCGC G^CGC
KpnI 1277 GGTACC GGTAC^C
MaeI 115 CTAG C^TAG
MaeII 1138 ACGT A^CGT
MluNI 1028 TGGCCA TGG^CCA
MroI 217 TCCGGA T^CCGGA
MscI 1028 TGGCCA TGG^CCA
NcoI 956 CCATGG C^CATGG
NspI 722 ACATGT [GA]CATG^[CT]
PaeR7I 562 CTCGAG C^TCGAG
PflMI 415 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 201 AGGACCT [GA]G^G[AT]CC[CT]
StuI 1168 AGGCCT AGG^CCT
StyI 956 CCATGG C^C[AT][AT]GG
Van91I 415 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 562 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 1118 GTAC GT^AC
1278 GTAC
AvaII 202 GGACC G^G[AT]CC
854 GGACC
BanI 452 GGCACC G^G[CT][GA]CC
1277 GGTACC
BanII 550 GAGCCC G[GA]GC[CT]^C
1089 GGGCCC
BglII 144 AGATCT A^GATCT
929 AGATCT
BsmFI 429 GGGAC GGGAC(10/14)
1102 GTCCC
BstAPI 285 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
357 GCAAGGTCTGC
BstXI 225 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
441 CCAGGCATTTGG
DsaI 59 CCACGG C^C[GA][CT]GG
956 CCATGG
HphI 3 GGTGA GGTGA(8/7)
972 GGTGA
PstI 160 CTGCAG CTGCA^G
364 CTGCAG
RsaI 1118 GTAC GT^AC
1278 GTAC
SfcI 160 CTGCAG C^T[GA][CT]AG
364 CTGCAG
SmlI 562 CTCGAG C^T[CT][GA]AG
733 CTCAAG
Tsp45I 152 GTGAC ^GT[GC]AC
557 GTGAC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI TspRI TthHB8I
XhoII