Restriction map for
pFN21AE2260
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanI BanII BbeI
BbsI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 1084 GTGCAC G^TGCAC
AocI 1118 CCTCAGG CC^T[ACGT]AGG
ApaLI 1084 GTGCAC G^TGCAC
AviII 743 TGCGCA TGC^GCA
BbrPI 1393 CACGTG CAC^GTG
BbvCI 1525 GCTGAGG CCTCAGC(-5/-2)
BciVI 909 GGATAC GTATCC(6/5)
BcnI 498 CCCGG CC^[GC]GG
BfaI 1285 CTAG C^TAG
BpmI 895 CTCCAG CTGGAG(16/14)
BsaAI 1393 CACGTG [CT]AC^GT[GA]
BsmAI 1016 GAGAC GTCTC(1/5)
BsrBI 721 GAGCGG CCGCTC(-3/-3)
BsrFI 842 ACCGGC [GA]^CCGG[CT]
BstYI 1538 AGATCC [GA]^GATC[CT]
Bsu36I 1118 CCTCAGG CC^T[ACGT]AGG
Cfr10I 842 ACCGGC [GA]^CCGG[CT]
DraI 850 TTTAAA TTT^AAA
DraII 58 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1393 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 83 CCATGG C^C[GA][CT]GG
Eco81I 1118 CCTCAGG CC^T[ACGT]AGG
EcoO109I 58 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 370 GATATC GAT^ATC
FspI 743 TGCGCA TGC^GCA
HindIII 1510 AAGCTT A^AGCTT
MaeI 1285 CTAG C^TAG
MflI 1538 AGATCC [GA]^GATC[CT]
MseI 851 TTAA T^TAA
NciI 498 CCCGG CC^[GC]GG
NcoI 83 CCATGG C^CATGG
PleI 1018 GACTC GAGTC(4/5)
PmaCI 1393 CACGTG CAC^GTG
PmlI 1393 CACGTG CAC^GTG
PpuMI 58 GGGTCCT [GA]G^G[AT]CC[CT]
SbfI 1269 CCTGCAGG CCTGCA^GG
SmlI 178 CTTGAG C^T[CT][GA]AG
Sse8387I 1269 CCTGCAGG CCTGCA^GG
StuI 302 AGGCCT AGG^CCT
Tru9I 851 TTAA T^TAA
XbaI 1284 TCTAGA T^CTAGA
XcmI 315 CCAGGCTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1538 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 256 GTCTAC GT^[AC][GT]AC
1435 GTCTAC
AcsI 187 GAATTC [GA]^AATT[CT]
411 GAATTC
ApoI 187 GAATTC [GA]^AATT[CT]
411 GAATTC
Asp700I 411 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1507 GAAAAGCTTC
BcgI 970 CGATCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1173 GCAGCTGGTTCG
BglI 1116 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1268 GCCTGCAGGGC
BsgI 531 CTGCAC GTGCAG(16/14)
954 CTGCAC
CfoI 744 GCGC GCG^C
822 GCGC
EcoRI 187 GAATTC G^AATTC
411 GAATTC
FauI 792 CCCGC CCCGC(4/6)
732 GCGGG
HhaI 744 GCGC GCG^C
822 GCGC
HinP1I 744 GCGC G^CGC
822 GCGC
HphI 1398 GGTGA GGTGA(8/7)
1079 TCACC
PvuII 873 CAGCTG CAG^CTG
1174 CAGCTG
TaqI 419 TCGA T^CGA
558 TCGA
Tsp509I 188 AATT ^AATT
412 AATT
TthHB8I 419 TCGA T^CGA
558 TCGA
XmnI 411 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1507 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I TspRI