Restriction map for
pFN21AE4387
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 728 GGTACC G^GTACC
AccI 511 GTCTAC GT^[AC][GT]AC
AccII 116 CGCG CG^CG
AcyI 365 GGCGCC G[GA]^CG[CT]C
ApaI 1114 GGGCCC GGGCC^C
Asp718I 728 GGTACC G^GTACC
AvaII 132 GGACC G^G[AT]CC
BanII 1114 GGGCCC G[GA]GC[CT]^C
BbeI 365 GGCGCC GGCGC^C
BbsI 619 GTCTTC GAAGAC(2/6)
BbvCI 871 CCTCAGC CCTCAGC(-5/-2)
BpuAI 619 GTCTTC GAAGAC(2/6)
BsaHI 365 GGCGCC G[GA]^CG[CT]C
BseRI 406 CTCCTC GAGGAG(10/8)
BsmBI 48 CGTCTC CGTCTC(1/5)
BsmFI 804 GGGAC GGGAC(10/14)
BsmI 265 GCATTC GAATGC(1/-1)
BspHI 680 TCATGA T^CATGA
BssHII 115 GCGCGC G^CGCGC
BstUI 116 CGCG CG^CG
DdeI 872 CTCAG C^T[ACGT]AG
DraI 999 TTTAAA TTT^AAA
DraII 131 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 788 CCGTGG C^C[GA][CT]GG
EcoO109I 131 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1060 GAATTC G^AATTC
EcoRV 1193 GATATC GAT^ATC
EcoT14I 1110 CCTTGG C^C[AT][AT]GG
HaeII 365 GGCGCC [GA]GCGC^[CT]
Hin1I 365 GGCGCC G[GA]^CG[CT]C
KasI 365 GGCGCC G^GCGCC
KpnI 728 GGTACC GGTAC^C
MvnI 116 CGCG CG^CG
NarI 365 GGCGCC GG^CGCC
PpuMI 131 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1156 CTGCAG CTGCA^G
RcaI 680 TCATGA T^CATGA
SexAI 626 ACCTGGT A^CC[AT]GGT
SfoI 365 GGCGCC GGC^GCC
SpeI 594 ACTAGT A^CTAGT
StyI 1110 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 280 GAATTT [GA]^AATT[CT]
1060 GAATTC
AlwI 159 GATCC GGATC(4/5)
1161 GATCC
AlwNI 874 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1159 CAGATCCTG
ApoI 280 GAATTT [GA]^AATT[CT]
1060 GAATTC
BalI 68 TGGCCA TGG^CCA
585 TGGCCA
BcnI 87 CCCGG CC^[GC]GG
96 CCGGG
BpmI 1018 CTCCAG CTGGAG(16/14)
940 CTGGAG
BsmAI 1045 GAGAC GTCTC(1/5)
49 GTCTC
BsrDI 108 CATTGC GCAATG(2/0)
1139 CATTGC
BstYI 158 AGATCC [GA]^GATC[CT]
1160 AGATCC
EaeI 68 TGGCCA [CT]^GGCC[GA]
585 TGGCCA
EarI 208 CTCTTC CTCTTC(1/4)
1210 CTCTTC
HapII 88 CCGG C^CGG
96 CCGG
HpaII 88 CCGG C^CGG
96 CCGG
Ksp632I 208 CTCTTC CTCTTC(1/4)
1210 CTCTTC
MflI 158 AGATCC [GA]^GATC[CT]
1160 AGATCC
MluNI 68 TGGCCA TGG^CCA
585 TGGCCA
MscI 68 TGGCCA TGG^CCA
585 TGGCCA
MslI 444 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1036 CACCAGAGTG
MspI 88 CCGG C^CGG
96 CCGG
NciI 87 CCCGG CC^[GC]GG
96 CCGGG
PleI 917 GACTC GAGTC(4/5)
1208 GACTC
PvuII 389 CAGCTG CAG^CTG
1021 CAGCTG
SfaNI 483 GCATC GCATC(5/9)
797 GCATC
SfcI 386 CTACAG C^T[GA][CT]AG
1156 CTGCAG
TaqI 1191 TCGA T^CGA
1206 TCGA
TthHB8I 1191 TCGA T^CGA
1206 TCGA
XhoII 158 AGATCC [GA]^GATC[CT]
1160 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BclI BfaI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI