##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccI          397  GTCTAC               GT^[AC][GT]AC
AlwNI         451  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          242  CTCGAG               C^[CT]CG[GA]G
AvaII         237  GGACC                G^G[AT]CC
BbvI          334  GCAGC                GCAGC(8/12)
BsaJI         233  CCAAGG               C^C[ACGT][ACGT]GG
BseRI         537  GAGGAG               GAGGAG(10/8)
BsiYI          27  CCTCATCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           27  CCTCATCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         242  CTCGAG               C^[CT]CG[GA]G
BsrDI         405  CATTGC               GCAATG(2/0)
DraI          166  TTTAAA               TTT^AAA
EcoT14I       233  CCAAGG               C^C[AT][AT]GG
Fnu4HI        334  GCAGC                GC^[ACGT]GC
ItaI          334  GCAGC                GC^[ACGT]GC
MwoI          474  GCTCTACCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        161  CATG                 CATG^
NlaIV         237  GGACCC               GG[ACGT]^[ACGT]CC
PaeR7I        242  CTCGAG               C^TCGAG
PleI          453  GACTC                GAGTC(4/5)
PpuMI         236  AGGACCC              [GA]G^G[AT]CC[CT]
SfaNI         139  GATGC                GCATC(5/9)
StyI          233  CCAAGG               C^C[AT][AT]GG
TfiI          216  GAATC                G^A[AT]TC
TseI          334  GCAGC                G^C[AT]GC
TspRI         256  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          242  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          438  AGCT                 AG^CT
              473  AGCT                
AlwI          265  GGATC                GGATC(4/5)
              492  GGATC               
BssKI          18  CCTGG                ^CC[ACGT]GG
               33  CCTGG               
BstNI          18  CCTGG                CC^[AT]GG
               33  CCTGG               
DraII          14  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              236  AGGACCC             
EcoO109I       14  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              236  AGGACCC             
EcoRII         18  CCTGG                ^CC[AT]GG
               33  CCTGG               
HaeIII         16  GGCC                 GG^CC
               63  GGCC                
HinfI         216  GAATC                G^A[ACGT]TC
              453  GACTC               
MseI           92  TTAA                 T^TAA
              167  TTAA                
MvaI           18  CCTGG                CC^[AT]GG
               33  CCTGG               
ScrFI          18  CCTGG                CC^[ACGT]GG
               33  CCTGG               
SmlI          242  CTCGAG               C^T[CT][GA]AG
              487  CTCAAG              
Tru9I          92  TTAA                 T^TAA
              167  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AfaI        BsrI        BstF5I      Cfr13I      DpnII       
FokI        MboI        MboII       MnlI        NdeII       
RsaI        Sau3AI      Sau96I      TaqI        TthHB8I