Restriction map for
pFN21AE5148
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# NO CUT ENZYMES ( 201 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
HphI KasI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 11 GGTACC G^GTACC
AfaI 12 GTAC GT^AC
Asp718I 11 GGTACC G^GTACC
AvaII 42 GGACC G^G[AT]CC
BanI 11 GGTACC G^G[CT][GA]CC
BanII 115 GGGCTC G[GA]GC[CT]^C
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
BbvI 23 GCAGC GCAGC(8/12)
BmyI 115 GGGCTC G[AGT]GC[ACT]^C
BsaI 64 GAGACC GGTCTC(1/5)
BsmAI 64 GAGAC GTCTC(1/5)
BsmFI 35 GGGAC GGGAC(10/14)
Bsp1286I 115 GGGCTC G[AGT]GC[ACT]^C
BsrDI 74 GCAATG GCAATG(2/0)
BsrI 99 ACTGG ACTGG(1/-1)
BssKI 15 CCTGG ^CC[ACGT]GG
BstF5I 123 CATCC GGATG(2/0)
BstNI 15 CCTGG CC^[AT]GG
Cac8I 167 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 165 GCGC GCG^C
DdeI 130 CTCAG C^T[ACGT]AG
DpnII 87 GATC ^GATC
DraII 80 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 80 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 15 CCTGG ^CC[AT]GG
EcoT14I 29 CCAAGG C^C[AT][AT]GG
FokI 123 CATCC GGATG(9/13)
HhaI 165 GCGC GCG^C
HinP1I 165 GCGC G^CGC
HinfI 140 GATTC G^A[ACGT]TC
KpnI 11 GGTACC GGTAC^C
MaeII 38 ACGT A^CGT
MboI 87 GATC ^GATC
MspA1I 144 CCGCTG C[AC]G^C[GT]G
MvaI 15 CCTGG CC^[AT]GG
NdeII 87 GATC ^GATC
RsaI 12 GTAC GT^AC
Sau3AI 87 GATC ^GATC
ScrFI 15 CCTGG CC^[ACGT]GG
StyI 29 CCAAGG C^C[AT][AT]GG
TfiI 140 GATTC G^A[AT]TC
TseI 23 GCAGC G^C[AT]GC
XcmI 29 CCAAGGGGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
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AciI 144 CCGC CCGC(-3/-1)
20 GCGG
BsaJI 15 CCTGGG C^C[ACGT][ACGT]GG
29 CCAAGG
BseRI 118 CTCCTC GAGGAG(10/8)
127 CTCCTC
BsiYI 27 CCCCAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCGCTGTGGGG
BslI 27 CCCCAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCGCTGTGGGG
Cfr13I 42 GGACC G^G[ACGT]CC
81 GGGCC
Fnu4HI 20 GCGGC GC^[ACGT]GC
23 GCAGC
HaeIII 82 GGCC GG^CC
102 GGCC
ItaI 20 GCGGC GC^[ACGT]GC
23 GCAGC
Sau96I 42 GGACC G^G[ACGT]CC
81 GGGCC
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
MboII MnlI MwoI NlaIV