Restriction map for
pFN21AE9625
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 165 CGCG CG^CG
AgeI 265 ACCGGT A^CCGGT
AocI 756 CCTTAGG CC^T[ACGT]AGG
AseI 1455 ATTAAT AT^TAAT
AsnI 1455 ATTAAT AT^TAAT
AvaI 1663 CTCGGG C^[CT]CG[GA]G
BanII 493 GAGCTC G[GA]GC[CT]^C
BciVI 1348 GTATCC GTATCC(6/5)
BclI 1503 TGATCA T^GATCA
BlpI 403 GCTCAGC GC^T[ACGT]AGC
BpmI 907 CTCCAG CTGGAG(16/14)
Bpu1102I 403 GCTCAGC GC^T[ACGT]AGC
BsaAI 256 CACGTA [CT]AC^GT[GA]
BsaWI 265 ACCGGT [AT]^CCGG[AT]
BsgI 1888 CTGCAC GTGCAG(16/14)
BsmI 1759 GAATGC GAATGC(1/-1)
BsoBI 1663 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1637 TGTACA T^GTACA
BsrDI 1641 CATTGC GCAATG(2/0)
BsrFI 265 ACCGGT [GA]^CCGG[CT]
BsrGI 1637 TGTACA T^GTACA
BssHII 164 GCGCGC G^CGCGC
BstAPI 1881 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 165 CGCG CG^CG
BstXI 584 CCAGCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 756 CCTTAGG CC^T[ACGT]AGG
CelII 403 GCTCAGC GC^T[ACGT]AGC
Cfr10I 265 ACCGGT [GA]^CCGG[CT]
DraIII 1366 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 304 GACTCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 756 CCTTAGG CC^T[ACGT]AGG
EcoRI 142 GAATTC G^AATTC
EcoT14I 528 CCTTGG C^C[AT][AT]GG
FbaI 1503 TGATCA T^GATCA
MaeII 257 ACGT A^CGT
MslI 422 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 165 CGCG CG^CG
PinAI 265 ACCGGT A^CCGGT
PshBI 1455 ATTAAT AT^TAAT
PstI 1859 CTGCAG CTGCA^G
SacI 493 GAGCTC GAGCT^C
SapI 2309 GAAGAGC GCTCTTC(1/4)
SphI 155 GCATGC GCATG^C
SspBI 1637 TGTACA T^GTACA
SspI 471 AATATT AAT^ATT
StyI 528 CCTTGG C^C[AT][AT]GG
TaqI 1048 TCGA T^CGA
TthHB8I 1048 TCGA T^CGA
XbaI 921 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 1539 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2131 GAAGAGGTTC
BbsI 202 GAAGAC GAAGAC(2/6)
651 GAAGAC
BpuAI 202 GAAGAC GAAGAC(2/6)
651 GAAGAC
BseRI 94 GAGGAG GAGGAG(10/8)
646 GAGGAG
BspMI 212 ACCTGC ACCTGC(4/8)
1881 GCAGGT
CfoI 164 GCGC GCG^C
166 GCGC
DraI 465 TTTAAA TTT^AAA
1140 TTTAAA
FauI 87 GCGGG CCCGC(4/6)
1779 GCGGG
HgaI 408 GCGTC GACGC(5/10)
1683 GCGTC
HhaI 164 GCGC GCG^C
166 GCGC
HinP1I 164 GCGC G^CGC
166 GCGC
HindIII 718 AAGCTT A^AGCTT
2273 AAGCTT
NspI 155 GCATGC [GA]CATG^[CT]
1426 ACATGC
PleI 304 GACTC GAGTC(4/5)
1441 GAGTC
PvuII 2246 CAGCTG CAG^CTG
2315 CAGCTG
SfcI 1154 CTACAG C^T[GA][CT]AG
1859 CTGCAG
Tsp45I 13 GTGAC ^GT[GC]AC
394 GTCAC
XmnI 1539 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2131 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbvI
BcnI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI XhoII